【摘要】 目的:探究磷脂酰肌醇-3激酶δ的表达与妊娠期糖尿病(GDM)的相关性,为临床治疗GDM提供理论依据。方法:选取2012年1月~2013年7月在河北医科大学第二医院行产前检查的孕妇166例,其中正常孕妇44例为NGT组、GDM孕妇122例为GDM组,测量并记录两组孕妇年龄、身高、体重、孕周,并计算BMI指数;采集孕妇的血液样品并收集血液中的白细胞,通过生化实验检测血糖、血脂、空腹胰岛素(FIN)、胆固醇、C反应蛋白(CRP)、高密度脂蛋白(HDL)等指标水平,并计算稳态模型β细胞功能指数(HOMA-B)以及胰岛素抵抗指数(HOMA-IR);采用RT-PCR方法检测PIK3CD mRNA在白细胞内的表达水平。结果:两组孕妇年龄、身高、体重、孕周、BMI值等指标比较,差异无统计学意义(P>0.05),GDM组HDL-C含量明显低于NGT组,空腹1 h和2 h后的葡萄糖浓度(FPG)及糖化血红蛋白(HbA1C)含量均明显高于NGT组,两组比较,差异有统计学意义(P<0.05);两组血甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、血清总胆固醇(TC)、CRP、HOMA-IR以及HOMA-B等指标比较,差异无统计学意义(P>0.05)。GDM组白细胞内的PIK3CD mRNA是NGT组的1.92倍。结论:GDM患者白细胞内的PIK3CD过量表达可引起HDL-C含量减少,并导致血糖浓度增加。更多还原

【Abstract】 Objective:To explore the correlation between phosphatidylinositol 3-kinase δ expression and gestational diabetes mellitus(GDM),provide a theoretical basis for clinical treatment of GDM. Methods:A total of 166 pregnant women receiving prenatal examination in the Second Hospital of Hebei Medical University from January 2012 to July 2013 were selected and divided into normal pregnancy group(44 pregnant women) and GDM group(122 GDM pregnant women); maternal ages,body heights,body weights and gestational weeks in the two groups were measured and recorded,body mass index(BMI) values in the two groups were calculated; the blood samples of pregnant women were abstracted and white blood cells were collected; blood glucose levels,blood lipids levels,fasting insulin levels,cholesterol levels,C-reactive protein(CRP) levels and high-density lipoprotein cholesterin(HDL-C) levels in the two groups were detected by biochemical tests; HOMA-β and HOMA-IR were calculated; RT-PCR was used to detect the expression level of PIK3 CD mRNA in white blood cells. Results:There was no statistically significant difference in maternal age,body height,body weight,gestational weeks and BMI between the two groups(P > 0. 05),HDL-C level in GDM group was statistically significantly lower than that in normal pregnancy group(P < 0. 05),one-hour and two-hour fasting blood glucose and glycosylated hemoglobin(HbA1C) levels in GDM group were statistically significantly higher than those in normal pregnancy group(P < 0. 05); there was no statistically significant difference in levels of triglyceride,low-density lipoprotein cholesterin(LDL-C),total cholesterol,CRP,HOMA-IR and HOMA-β between the two groups(P >0.05). PIK3 CD mRNA in white blood cells of GDM group was 1.92 times more than that of normal pregnancy group. Conclusion:Over-expression of PIK3 CD in white blood cells of GDM patients can induce decrease of HDL-C level and increase of blood glucose level.更多还原