【摘要】 目的研究蒲桃(原变种)Syzygium jambos var.jambos茎的化学成分。方法利用硅胶、葡聚糖凝胶、氧化铝和制备液相等各种色谱技术进行分离和纯化,根据波谱数据分析鉴定化合物结构;利用MTT法评价分离化合物对人肝癌细胞Huh-7细胞毒作用。结果从蒲桃茎醋酸乙酯和甲醇提取物中分离得到12个化合物,分别鉴定为麦珠子酸(1)、urolithin A(2)、邻苯二甲酸二正丁酯(3)、桦木酸(4)、熊果酸(5)、1-(4-methoxyphenyl)-1,2-propanediol(6)、2,6-dimethoxy-1,4-benzoquinone(7)、邻苯二甲酸二异丁酯(8)、β-谷甾醇(9)、3-乙酰-熊果酸(10)、积雪草酸(11)和阿江榄仁酸(12)。细胞活性测试结果表明在50μmol/L浓度时化合物1、7、10、11+12混合物对Huh-7细胞均未表现出显著的细胞毒作用。结论除化合物4,其他11个化合物均为首次从植物分离获得,所测试化合物均无明显细胞毒活性。更多还原

【Abstract】 Objective To study the chemical constituents from the stems of Syzygium jambos var. jambos. Methods The phyotchemicals were isolated by using various chromatographic techniques such as silica gel, Sephadex LH-20, alumina column chromatography, and preparative HPLC process. The structures were elucidated on the basis of spectral data analysis. Their cytotoxicity against human liver cancer cells Huh-7 was conducted using MTT assay. Results Twelve compounds were isolated from the EtOAc and MeOH extracts in the stems of S. jambos and identified as alphitolic acid(1), urolithin A(2), dibutyl phthalate(3), betulinic acid(4), ursolic acid(5), 1-(4-methoxyphenyl)-1, 2-propanediol(6), 2, 6-dimethoxy-1, 4-benzoquinone(7), diisobutyl phthalate(8), β-sitosterol(9), 3-acetyl-ursolic acid(10), asiatic acid(11), and arjunolic acid(12), respectively. All tested compounds 1, 7, 10, and 11 + 12 mixture did not show the cytotoxic activities towards human tumor cells Huh-7 in the test range up to 50 μmol/L. Conclusion Except compound 4, the other 11 compounds are obtained from this plant for the first time. All tested compounds exhibit no significant cytotoxic activity against Huh-7 cells.更多还原