【摘要】 目的:研究川崎病患儿细胞免疫功能状况及转录因子T-bet和GATA3的调控作用。方法:选取41例川崎病患儿为研究对象,同时选取年龄和性别相匹配的因急性上呼吸道感染发热儿童40例为对照组,分别采用三色荧光流式细胞术和半定量逆转录-聚合酶链反应检测2组患儿外周血Th1、Th2细胞数量及单个核细胞中T-bet和GATA3 mRNA水平。结果:川崎病患儿外周血Th1、Th2细胞比率及单个核细胞转录因子T-bet mRNA、GATA3 mRNA转录水平分别为(10.36±3.69)%、(6.46±2.28)%、0.51±0.16和0.38±0.13,均显著低于对照组[(25.26±5.22)%、(16.87±4.35)%、0.62±0.21和0.46±0.12,P<0.05]。Spearman相关分析显示Th1和Th2细胞比率分别与T-bet mRNA和GATA3 mRNA水平呈正相关。结论:川崎病患儿急性期T细胞并非活化而是处于抑制状态,T-bet和GATA3分别对Th1和Th2细胞增殖具有调控作用。更多还原

【Abstract】 AIM:To clarify the functional state of T cells and the role of transcription factors T-bet and GATA3 in choldren with Kawasaki disease.METHODS:The patients with acute Kawasaki disease(n = 41) were enrolled as experimental group and 40 febrile children with acute upper respiratory tract infection were selected as control group.The mRNA expression levels of T-bet and GATA3,and the percentages of Th1 and Th2 cells were examined by RT-PCR and flow cytometry respectively.RESULTS:The mRNA expression levels of T-bet and GATA3 and the percentages of Th1 and Th2 cells in Kawasaki disease group were 0.51 ± 0.16,0.38 ± 0.13,(10.36 ± 3.69) % and(6.46 ± 2.28) %,respectively,and were significantly lower than those in control group [0.62 ± 0.21,0.46 ± 0.12,(25.26 ± 5.22) % and(16.87 ±4.35) %,P <0.05].There were positive correlations between the mRNA expression levels of T-bet and GATA3,and the percentages of Th1 and Th2 cells.CONCLUSION:Associated with the suppression of both T-bet and GATA3 mRNA expression,the function of Th1 and Th2 cells are suppressed simultaneously in acute Kawasaki disease.更多还原