【摘要】 目的:探讨负向调控miR-9对人鼻咽癌细胞增殖、迁移和侵袭作用。方法:用脂质体LipofectamineTM2000转染合成抑制剂的方法抑制鼻咽癌细胞miR-9表达,转染抑制对照剂作为对照组。CCK-8法和流式细胞术检测细胞增殖和细胞周期变化;Transwell侵袭实验和划痕实验检测细胞侵袭和迁移能力;免疫印迹实验检测蛋白变化。结果:抑制鼻咽癌细胞miR-9表达后,肿瘤增殖能力降低(P<0.05),G0/G1期细胞增多[CNE2:(57.96±1.39)%vs(47.93±1.76)%,P<0.05;CNE1:(51.24±0.88)%vs(48.29±0.39)%,P<0.05],迁移距离明显缩短[CNE2:(186.50±7.94)μm vs(247.56±15.56)μm,P<0.05;CNE1:(139.06±16.73)μm vs(230.66±14.27)μm,P<0.01],CNE2细胞中侵袭细胞数明显减少(43.00±3.17 vs 65.80±5.20,P<0.01),β-连环蛋白(β-catenin)表达被抑制。结论:在鼻咽癌细胞中,负向调控miR-9可抑制鼻咽癌细胞的增殖、侵袭和迁移。更多还原

【Abstract】 AIM:To investigate the effects of down-regulated miR-9 expression on the proliferation,invasion and migration of nasopharyngeal carcinoma(NPC) cells.METHODS:Human NPC CNE1 and CNE2 cells were transfected with the inhibitor of miR-9 by Lipofectamine to down-regulate the expression of miR-9,and the cells transfected with an inhibitor control were also set up.The cell proliferation and cell cycle were evaluated by CCK-8 assay and flow cytometry.The cell invasion and migration abilities were detected by Transwell invasion and wound-healing assays.Immunoblotting was applied to analyze the levels of the proteins.RESULTS:Compared with control group,inhibition of miR-9 expression in the NPC cells by transfection of the miR-9 inhibitor significantly decreased the proliferation ability(P < 0.05).The percentages of the cells in G0/ G1phase [CNE2:(57.96 ± 1.39) % vs(47.93 ± 1.76) %,P < 0.05;CNE1:(51.24 ± 0.88) % vs(48.29 ± 0.39) %,P < 0.05] were significantly increased.The migration distances [CNE2:(186.50 ± 7.94) μm vs(247.56 ± 15.56) μm,P < 0.05;CNE1:(139.06 ± 16.73) μm vs(230.66 ± 14.27) μm,P < 0.01] and the invasion ability of the CNE2 cells(43.00 ± 3.17 vs 65.80 ± 5.20,P < 0.01) were also significantly inhibited.Moreover,the tumor cells transfected with the inhibitors produced lower β-catenin.CONCLUSION:Inhibition of miR-9 expression suppresses the proliferation,invasion and migration of nasopharyngeal carcinoma cells.更多还原