【摘要】 目的:探讨葡萄球菌肠毒素A(staphylococcal enterotoxin A,SEA)对甲磺酸伊马替尼(imatinib mesylate,IM)抑制T淋巴细胞活化的影响。方法:2 mg/L SEA和50 nmol/L IM联合作用于Jurkat细胞24 h后,用实时荧光定量PCR技术检测CD3ε和ζ链mRNA表达水平变化;Western blotting技术检测CD3ε和ζ链蛋白水平变化。结果:单纯IM组CD3ε和ζ链mRNA及蛋白表达均表现下调;SEA与IM联合用药组可以逆转IM下调CD3ε和ζ链mRNA及蛋白表达水平作用。SEA拮抗IM抑制作用中,对CD3ε链mRNA表达上调的作用明显大于CD3ζ链。结论:SEA能拮抗IM对T细胞CD3ε和ζ链表达的抑制作用。更多还原

【Abstract】 AIM: To investigate the effects of staphylococcal enterotoxin A( SEA) on the inhibition of T lymphocyte activation induced by imatinib mesylate( IM). METHODS: Jurkat cells were stimulated with SEA( 2 mg / L) and IM( 5 nmol / L) for 24 h. The mRNA expression of CD3ε and ζ chains was measured by real-time fluorescence quantitative PCR. The protein levels of CD3ε and ζ chains were detected by Western blotting. RESULTS: The expression of CD3ε and ζ chains at mRNA and protein levels was down-regulated in the Jurkat cells stimulated by IM alone. These down-regulations of CD3ε and ζ chains were reversed by the stimulation of IM combined with SEA. The antagonistic effect of SEA on IM-mediated inhibition of CD3ε mRNA expression was significantly greater than that on CD3ζ mRNA. CONCLUSION: SEA antagonizes imatinib-mediated inhibitory effect on T cell activation.更多还原