【摘要】 目的探讨建立一种稳定、操作性好的大鼠原位肾移植模型的外科技术及其改进方法。方法用4℃肝素生理盐水原位灌注供肾,手术显微镜下行供肾动脉、静脉、输尿管与受鼠同名血管及输尿管端端吻合。受鼠双侧肾脏一起切除,建立大鼠左侧原位肾移植模型。结果经过技术改进,建立了稳定、易行的适合器官移植免疫学研究所需的大鼠单侧原位肾移植模型,模型成功率可达80%以上。对已完成的10对大鼠移植时间进行统计,完成1对大鼠肾移植需要(211.00±13.24)分钟,其中供肾切取(87.00±5.34)分钟,供肾移植(120.00±7.90)分钟;供肾移植中原肾脏切除(27.00±1.12)分钟,动脉吻合(35.00±1.98)分钟,静脉吻合(36.00±3.26)分钟,输尿管吻合(22.00±1.54)分钟。供肾热缺血时间为30秒,肾脏冷缺血时间为100分钟。10对原位肾移植大鼠中8对大鼠存活时间大于3天,移植肾形态、功能良好,其余2对大鼠死亡原因包括吻合口出血、血栓形成、尿漏等。结论本文所述方法和改进技巧有利于建立一种稳定的、适用于器官移植免疫学研究的大鼠原位肾移植模型。更多还原

【Abstract】 Objective To investigate the procedure improvement of building a stable and operational orthotopic renal transplantation model in rats. Methods The left kidney was removed from the donor rat after perfusing it with ice-cold heparinized saline through the abdominal aorta. The donor kidney was transplanted orthotopically into a bilaterally nephrectomized recipient by end-to-end anastomosis of the left renal vessels and the left ureter using microsurgical technique. Results According to the improved procedure, a stable and easily operational renal transplantation model was set up with a success rate above 80%. 10 pairs of rats have received renal transplantation surgeries till now. The mean total surgery time was(211.00±13.24)minutes, with donor nephrectomy of(87.00±5.34)minutes and renal transplantation of(120.00±7.90)minutes. During the transplantation surgery, the time of nephrectomies was(27.00±1.12)minutes, with artery anastomosis of(35.00±1.98)minutes, vein anastomosis of(36.00±3.26)minutes, and ureteroureterostomy of(22.00±1.54)minutes. The mean warm ischemia time was 30 seconds, and the mean cold ischemia time was 100 minutes. 3-day survival rate was 80% with good graft function. 2 recipients died of anastomotic bleeding, thrombosis, urine leakage, and so on. Conclusion With the procedure improvement, it was very helpful to set up a stable and operational rat renal transplantation model for immunological researches.更多还原