【摘要】 目的建立以高效液相色谱法测定银杏叶缓释微丸胶囊中山奈素、异鼠李素、槲皮素含量的方法。方法以C18为色谱柱,流动相为甲醇-0.4%磷酸溶液(45∶55),检测波长为360 nm,流速为1 m L/min,柱温为30℃,进样量是10μL。结果槲皮素在0.1505~0.9030μg(r=0.9999)浓度范围内有良好的线性关系,平均回收率为99.86%,RSD=0.4%;山奈素在0.1450~0.8940μg(r=0.9998)浓度范围内有良好的线性关系,平均回收率为99.79%,RSD=0.5%;异鼠李素在0.0995~0.5970μg(r=0.9998)浓度范围内有良好的线性关系,平均回收率为99.80%,RSD=0.3%。结论本方法简便、准确、专属性强,可用于该制剂的质量控制。更多还原

【Abstract】 Objective To establish a method for the determination of haempferol, isorhamnetin and quercetin in folium ginkgo pellets capsules MethodsHPLC was performed on a C18 column using mathanol-0.4%H3PO4(45∶55) as the mobile phase at a flow rate of 1 m L/min. The detection wavelength was at 360 nm. The column temperature was 30 ℃ and the sample size was 10 μL. Results The linear range of quercetin was 0.1505-0.9030 μg(r=0.9999), the average recovery was 99.86%(RSD=0.4%); the linear range of haempferol was 0.1450-0.8940 μg(r=0.9998), the average recovery was 99.79%(RSD=0.5%); the linear range of isorhamnetin was 0.0995-0.5970 μg(r=0.9998), the average recovery was 99.80%(RSD=0.3%). Conclusions The method is simple, accurate, and special and can be used for the quality control of folium ginkgo pellets capsules.更多还原