The microsporogenesis and mature pollens in different ploidy Phalaenopsis were observed using squash method,besides,DNA content of mature pollen was analyzed using flow cytometry. The results indicated that there were a few unreduced gametes including haploidy gametes in different ploidy Phalaenopsis. The mean diameter of haploidy,diploidy,triploidy and tetraploidy pollens was 11.4,17.2,19.1 and 20.3 μm,respectively,which was positively correlated with ploidy level(r = 0.935,P < 0.065). The occurrence frequ...
(1Guangdong Provincial Key Laboratory of Plant Molecular Breeding,South China Agricultural University,Guangzhou510642,China;2Guangzhou Flower Research Center,Guangzhou 510360,China)蝴蝶兰(Phalaenopsis)栽培品种多数为多倍体(庄东红等,2007;谢利等,2014),主要是