【摘要】 目的建立高效液相色谱法测定万古霉素的血药浓度。方法血浆样品经硫酸锌沉淀处理,采用反相高效液相色谱法,流动相为乙腈-0.02moL·L-1磷酸二氢钾缓冲液(8∶92,V/V),检测波长为236nm,流速为1.0 mL·min-1,柱温为25℃,进样量为10μL。采用峰面积外标法定量测定人血浆中的万古霉素浓度。结果万古霉素进样浓度在2.5~200 mg·L-1范围内与峰面积积分值呈良好的线性关系(r=0.9997,n=7);日内RSD为3.91%,2.38%,1.77%,日间RSD为4.01%,2.35%,1.82%;相对回收率分别为99.2%~100.3%;稳定性考察结果表明血浆样品在24h内稳定。结论本方法专属性强,简单方便,适用于万古霉素的血药浓度监测。更多还原

【Abstract】 Objective To establish an HPLC method for determination of plasma vancomycin. Methods The vancomycin in plasma extracted by the sedimentation with zinc sulfate was determined by an HPLC method with mobile phase consisted of a mixture of acetonitrile- potassium dihydrogen. Phosphate buffer solution(8∶92, V/V) at the flow rate of 1.0 mL·min-1. The detective wavelength was set at 236 nm and the column temperature was 25℃. The injection volume was 10μL. ResultsThe linear range of vancomycin was 2.5~200 mg·L-1(r=0.9997,n=7)with within-day RSD of 3.91%, 2.38%, 1.77% and betweenday RSD of 4.01%, 2.35%, 1.82%, the relevant recovery of 99.2%~100.3%.Stability investigation showed that the plasma sample with vancomycin were stable in 24 h. Conclusion The method is specific and simple, which can be used for the determination of plasma vancomycin.更多还原