【摘要】 目的采用高效液相色谱(HPLC)法测定不同产地芫花根皮中西瑞香素含量,确定何种产地芫花根皮中西瑞香素含量最高。方法采用Prodigy 5u 100A C18柱(250 mm×4.60 mm,5μm),流动相为甲醇-0.2%磷酸水溶液(55∶45),流速为1.0 m L/min,柱温30℃,紫外检测波长346 nm,进样量20μL。结果西瑞香素质量浓度在0.5~20μg/m L范围内与峰面积具有良好的线性关系(r=0.998 4),平均回收率为98.62%,RSD=4.08%(n=6)。不同产地芫花根皮药材中西瑞香素含量差异较大。结论该法可准确地对芫花根皮药材中有效成分西瑞香素进行定量定性测定,对于保证其内在质量和临床疗效具有重要的意义。更多还原

【Abstract】 Objective To establish a HPLC determination method for the daphnoretin content in the root bark from different habitats of Daphne genkwa in order to determine what habitat of the root bark of Daphne genkwa has the highest daphnoretin content. Methods The OSD column( 250 mm × 4. 60 mm,5 μm) was adopted with the mobile phase of methanol- 0. 2% phosphoric acid aqueous solution( 55 ∶ 45) at a flow rate of 1. 0 mL / min,the column temperature was 30 ℃ and the UV detection wavelength was 346 nm. The sample size was 20 μL. Results Daphnoretin in the range of 0. 5- 20 μg / mL had good linear relation( r = 0. 998 4), and the average recovery rate was 98. 62%,RSD = 4. 08%( n = 6). The daphnoretin content in the root bark of Daphne genkwa had large difference among the different habitats. Conclusion This method can accurately perform the quantitative and qualitative determination of the daphnoretin content in the root bark of Daphne genkwa and has a great significance for ensuring its internal quality and clinical curative effect.更多还原