【摘要】 目的建立测定总环烯醚萜类含量的方法,比较不同产地缬草中总环烯醚萜类的含量。方法采用分光光度法,以缬草中总环烯醚萜类与碱性羟胺-高氯酸铁显色剂产生显色反应,于波长约522 nm处测定吸光度,按缬草三酯来计算总环烯醚萜类含量。结果缬草三酯量在0.05~1.40 mg范围内与吸光度具有良好的线性关系,平均加样回收率98.50%,RSD=1.02%,不同产地缬草中总环烯醚萜类的含量有明显差异。结论该方法准确可靠,能更好地评价缬草的质量,提示缬草中总环烯醚萜类的含量与产地有关。更多还原

【Abstract】 Objective To determine and compare the contents of total iridoids in valerian from different origins.Methods The total iridoids in valerian was detected by reaction with alkaline hydroxylamine-perchloric acid iron reagent,and the absorbance was measured at 522 nm by spectrophotometry. Results The content of valepotriate and its absorption were linear within the range of 0. 05- 1. 40 mg · mL- 1. The average recovery was 98. 50%,and RSD was 1. 02%. There was significant difference between the contents of iridoid in valerian from different habitats. Conclusion This method is accurate and reliable for evaluating the quality of valerianand. The contents of valeriana is related to its habitats.更多还原