【摘要】 目的通过检测大脑中动脉梗死(MCAO)大鼠脑组织的内皮素(ET)-1、水通道蛋白4(AQP4)及水通道蛋白9(AQP9)含量的变化来研究镇肝熄风汤发挥神经保护作用的可能生物学基础。方法 MCAO术前给予大、小剂量镇肝熄风汤(3.0,1.5 g·mL-1)灌胃14 d,采用线栓法制备MCAO。评价镇肝熄风汤预处理对MCAO大鼠脑缺血后行为障碍及脑水肿程度的影响;应用免疫组化方法研究镇肝熄风汤预处理对MCAO大鼠脑组织ET-1的影响;采用western blot技术分析MCAO大鼠缺血侧脑组织AQP4与AQP9表达量的变化和镇肝熄风汤预处理对其的影响。结果预先使用镇肝熄风汤14 d,可明显改善MCAO大鼠行为学障碍,减轻脑水肿程度;MCAO大鼠缺血侧脑组织中ET-1蛋白表达量均有不同程度下降;明显增加MCAO大鼠缺血侧脑组织AQP4与AQP9蛋白表达量。结论镇肝熄风汤预处理可抑制ET-1表达,增加AQP4与AQP9表达,减轻脑水肿程度。更多还原

【Abstract】 Objective To study the neuroprotective effect of zhengan xifeng decoction( ZGXFD),a tranditional Chinese herbal compound,on conscious rats with middle cerebral artery occlusion( MCAO). Methods The rat model of focal cerebral ischemia was established with the suture-occuluded method and was treated with different doses of ZGXFD( 1. 5 and 3 g·mL- 1,respectively) before MCAO. The effect of ZGXFD on the behavior disorder and extent of cerebral edema of the MCAO rats were detected. ET-1 expression in the rat brain tissues was examined by immunohistochemistry method. The AQP 4 and AQP 9 expression were determined by Western blotting. Results After MCAO,different degrees of behavior disorder appeared in all rats. Pretreatment with ZGXFD improved the grade of behavior disorder of the rats,reduced the extent of cerebral edema,and increased AQP4 and AQP9 obviously. After MCAO,the tissue ET-1 expression was increased obviously but the ZGXFD could reverse it. Compared with the MCAO group,the AQP9 protein expression significantly increased after 6 h of hypoxia while the AQP4 did not change. Conclusion These findings suggest that ZGXFD can directly modulate ET-1,AQP4 and AQP9 expression and reduce cerebral edema.更多还原