【摘要】 目的探讨三氧化二砷(As2O3)对人脑胶质瘤SHG44细胞增殖的抑制作用及其作用机制。方法采用噻唑蓝(MTT)法观察As2O3对SHG44细胞的增殖抑制率;激光共聚焦显微镜检测As2O3作用于SHG44细胞后细胞内活性氧自由基(ROS)水平变化。结果 As2O3对SHG44细胞增殖具有明显抑制作用,随着As2O3浓度的升高和作用时间的延长,细胞的增殖抑制率升高,12μmol·L-1As2O3对SHG44细胞增殖的抑制率可达63.8%;As2O3可以明显提高SHG44细胞内ROS水平,其ROS水平随As2O3作用浓度的增高明显增高,具有浓度依赖关系。结论 As2O3能明显抑制SHG44细胞的增殖,抑制作用呈现剂量依赖性和时间依赖性,其机制与升高细胞内活性氧水平有关。更多还原

【Abstract】 Objective To investigate the anti-proliferation effects of arsenic trioxide( As 2 O 3) on human glioma cells-44( SHG44) in vitro and its mechanism. Methods The inhibitory ratio of the cells by As 2 O 3 was measured by MTT assay. The intracellular ROS were determined by a probe( DCFH-DA) under a laser scanning confocal microscopy. Results As 2 O 3 significantly inhibited SHG44 cells proliferation. The proliferation inhibition rate was dose-dependent and time-dependent. The proliferation inhibition by 12 μmol · L-1As 2 O 3 on SHG44 cells was as high as 63. 8%. After treatment with As 2 O 3,the intracellular ROS was increased markedly in a concentration-dependent manner. Conclusion As 2 O 3 can inhibit the proliferation of SHG44 cells in both dose-dependent and time-dependent manners. The inhibition was associated with elevation of intracellular ROS.更多还原