【摘要】 目的建立抗宫炎凝胶剂定性鉴别的研究方法 ,为制订其质量标准提供基础。方法采用高效液相色谱法对处方中的广东紫珠进行定性鉴别。采用薄层色谱法对处方中的乌药、益母草进行定性鉴别。乌药鉴别中以甲苯-乙酸乙酯(15∶1)为展开剂;益母草鉴别中以丙酮-无水乙醇-盐酸(10∶6∶1)为展开剂。结果高效液相色谱中广东紫珠在供试品色谱中呈现出与对照品色谱中保留时间相同的金石蚕苷色谱峰(t=5.51 min)。薄层色谱中可检出乌药、益母草,且阴性对照无干扰。结论本研究所采用的抗宫炎凝胶剂的定性鉴别方法简便可靠,可用于抗宮炎凝胶剂质量标准的研究。更多还原

【Abstract】 Objective To establish the qualitative identification of Kanggongyan Gels, and provide the foundation for the development of its quality standard. Methods HPLC qualitative idetification method of Callicarpa kwangtungensis in the prescription were performed, and TLC qualitative identification method of Leonurus and the root of three-nerved spicebush in the prescription were performed. The developer for Leonurus was methylbenzene-ethyl acetate(15 ∶1); for the root of three-nerved spicebush was acetone-absolute ethyl alcohol-hydrochloric acid(10 ∶6∶1). Results Callicarpa kwangtungensis showed the same Poliumoside chromatographic peak in the chromatogram of the sample solution and the reference solution at the same retention time(t = 5.51 min). Leonurus and the root of three-nerved spicebush could be detected by TLC and the negative control without interference. Conclusion The qualitative identification method in this study of Kanggongyan Gels is simple, reliable and specific with good reproducibility and can be used in the quality standard of Kanggongyan Gels for research.更多还原