【摘要】 目的研究间质干细胞早期定向成骨分化基因表达谱,为研究基因对早期成骨定向分化调控机制提供实验基础。方法分别提取重组人骨形成蛋白2(rhBMP-2)诱导组和对照组C3H10T1/2细胞总RNA,进行扩增标记后,与ArraySTAR小鼠基因芯片杂交,应用生物信息学软件GeneSpring和GATHER对基因芯片数据进行分析。应用STRING在线软件对差异表达基因构建蛋白互作网络并进行网络分析。结果 C3H10T1/2早期成骨分化中,主要富集发育、器官形成等分子功能本体以及细胞因子-细胞因子受体作用信号通路。成骨分化1 d和4 d均上调表达基因42个,下调表达基因45个。网络分析研究表明:Egfr、Cxcl12等信号分子参与调控rhBMP-2诱导成骨分化。结论筛选的差异表达基因和信号分子对早期成骨分化调控具有重要作用,为进一步全面解析早期成骨定向分化提供实验基础。更多还原

【Abstract】 Objective To investigate the gene expression profiles associated with early osteoblastic differentiation on mesenchymal stem cells, and to provide an experimental basis for the study of gene regulation mechanism for early osteoblastic differentiation. Methods The total RNA was extracted from C3H10T1/2 mesenchymal stem cells between control and rhBMP-2 osteoblastic differentiation group, and then cRNA was labeled with fluorescence labels and hybridized with ArraySTAR mouse genechip. The gene expression profiles were analyzed with bioinformatics software GeneSpring and GATHER. The protein interaction network of differentially expressed genes was constructed and analyzed by online software STRING. Results The molecular function gene ontology(GO) including development and organogenesis and cytokine-cytokine receptor interaction signaling pathway were mainly enriched during C3H10T1/2 early osteogenic differentiation. A total of 42 differentially expressed genes were upregulated and 45 genes were downregulated from rhBMP-2 induced osteoblast 1 and 4 days. Protein network analysis showed that Egfr, Cxcl12 were very important signaling molecules which involved in rhBMP-2 induced osteoblast. Conclusion The screening differentially expressed genes and signaling molecules have an important role for regulation of early osteoblastic differentiation and provide further experimental basis for comprehensive analysis of early osteoblastic differentiation.更多还原