【摘要】 目的观察Apelin-13对实验性自身免疫性神经炎(EAN)大鼠淋巴结中炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和干扰素-γ(IFN-γ)表达的影响。方法采用周围神经髓鞘抗原(P257-81)注射入Lewis大鼠后肢足垫诱导EAN模型。Lewis雄性大鼠随机分为对照组、EAN模型组和Apelin处理组。Apelin处理组于免疫当天至第15天每天尾静脉注射Apelin-13(0.1 mg/kg)。观察各组大鼠发病情况和坐骨神经组织病理学变化,采用实时定量PCR和Western blot检测淋巴结中TNF-α、IL-6和IFN-γ表达。结果与EAN模型组比较,Apelin处理组大鼠最初发病时间明显延长,高峰期临床评分显著降低,炎症因子TNF-α、IL-6和IFN-γ的表达显著降低(均P<0.05)。结论 Apelin-13对实验性自身免疫性神经炎大鼠有治疗作用,其机制可能与下调TNF-α、IL-6和IFN-γ的表达有关。更多还原

【Abstract】 Objective To observe the effects of Apelin-13 on the expression of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interferon-γ(IFN-γ) of lymph node in experimental autoimmune neuritis(EAN) rats. Methods The rats models of EAN were established by injection of peripheral nerve myelin sheath antigen(P257-81) in the foot pad of male Lewis rats. The rats were randomly divided into the control group, EAN model group, and Apelin treatment group. The rats in Apelin treatment group were injected with Apelin-13(0.1 mg/kg) by tail vein once daily from the 1st day to the 15th day. The clinical incidence and pathology in the rats were assessed. The expressions of TNF-α, IL-6 and IFN-γ in lymph node were measured by real-time PCR and Western blot respectively. Results Compared with the EAN model group, the initial time of nervous symptom significantly increased, the maximal neurological score significantly decreased, the expressions of TNF-α, IL-6 and IFN-γ in lymph node significantly decreased in Apelin treatment group(all P < 0.05). Conclusion Apelin-13 can treat the EAN in EAN rats model, the mechanism of which may be related with down-regulation of TNF-α, IL-6 and IFN-γ.更多还原