【摘要】 目的探明单核苷酸多态(SNPs)在人表皮生长因子前体蛋白(preproEGF)mRNA/cDNA序列中的分布状况。方法凭借美国生物信息中心(NCBI)平台和单核苷酸多态资料库(dbSNP)检索、分析、标注和图解相关SNPs在人preproEGF多肽mRNA/cDNA序列中的分布位点和生物信息学特征。结果分布在人肾源和其他组织源preproEGF多肽mRNA/cDNA序列中的SNPs总计有106个,其中84个同位SNPs大多数以外显子6~8、11~12、16~19和22~23为间隔并集中归位于第1~5、10、13~15、20~21、24外显子序列中,另外的22个非同位SNPs大多数以密集丛簇为特征而各自分布在两类序列3’端非编码序列中,但个别例外则单独归位于肾源类序列的第9外显子中。结论 SNPs在人两类preproEGF多肽mRNA/cDNA序列中的生物信息学分布、特征和图示对于SNP与疾病或性状的相关性研究及课题设计颇具参考价值。更多还原

【Abstract】 Objective To ascertain the distribution of single nucleotide polymorphisms(SNPs) in the mRNA/cDNA of human epidermal growth factor precursor(preproEGF).Methods By the web-based bioinformatics platform for NCBI tools,the dbSNP was searched and analyzed for SNPs relative to human preproEGF mRNA/cDNA.These SNPs were then made the annotation and the diagram for their position and distribution in mRNA/cDNA sequence.Results A total of 106 SNPs,of which 84 were considered the locus-same and 22 were considered the locus-different,were distributed in two mRNA/cDNA sequences with coding regions for preproEGFs from human kidney and other organic tissues.The majority of locus-same SNPs were clustered and assigned to exon 1-5,10,13-15,20-21,24 individually,which were spaced by exon 6-8,11-12,16-19,22-23 apart in preproEGF-coding regions of two mRNA/cDNA sequences.Most of locus-different SNPs were clustered and distributed in three prime non-coding regions of two mRNA/cDNA sequences respectively.However,a rather unique locus-different SNP was located in exon 9 of preproEGF mRNA/cDNA from human kidney.Conclusion The bioinformatics distribution and characterization that the illustration exhibits of SNPs in human preproEGF mRNA/cDNA sequences are useful to help the research design and the study in human disease associated with preproEGF.更多还原