【摘要】 目的探讨芍药苷对脂多糖(lipopolysaccharides,LPS)诱导H9C2细胞氧化应激损伤的作用。方法采用LPS刺激H9C2细胞来检测相应的氧化应激指标从而间接反映细胞损伤程度,通过检测细胞内活性氧团基(reactive oxygen species,ROS)水平的变化来观察芍药苷对其作用。本实验将细胞分为正常对照组、LPS氧化损伤组、LPS加芍药苷组(低、中、高剂量),按实验设计因素处理后,用荧光酶标仪及流式细胞仪检测细胞内ROS水平。结果芍药苷对H9C2细胞具有低毒性,它可以降低胞内ROS的水平,且在本实验使用浓度范围内,芍药苷使细胞内ROS降低呈浓度依赖性;在本实验设计的时间范围内,芍药苷使细胞内ROS下降呈时间依赖性。结论芍药苷对LPS诱导H9C2细胞氧化应激损伤具有明显的改善作用。更多还原

【Abstract】 Objective To investigate the effect of Paeoniflorin on H9C2 cell injury by LPS-induced oxidative stress. Methods H9C2 cells cultured in vitro were stimulated by LPS,and the corresponding parameters of oxidative stress which indirectly reflect the damage of cells were detected. The effect of paeoniflorin was observe by detecting the levels of intracellular reactive oxygen species(ROS). In this study,cells were divided into normal control group,LPS oxidative damage group,LPS and paeoniflorin groups(low,medium and high doses). Intracellular ROS levels were detected with microplate readers and fluorescence flow cytometry after dealing with experimental factors designed. Results Paeoniflorin on H9C2 cells had low toxicity. It can reduce the level of intracellular ROS. And in the concentration range used in this study,paeoniflorin which reduced intracellular ROS was concentration-dependant. In this experimental time range,the intracellular ROS fall was time-dependent. Conclusion Paeoniflorin improves the H9C2 cell injury by LPS-induced oxidative stress.更多还原