【摘要】 目的:应用响应面法优化茶多酚柔性脂质体的制备工艺参数,筛选出最佳处方。方法:以蛋黄卵磷脂(EPC)和胆固醇(CH)作为膜材,以胆酸钠作为柔软剂,采用逆向薄膜蒸发法制备茶多酚柔性脂质体。以脂质体的包封率为考察指标,采用三因素三水平Box-Behnken响应平面法考察脂质体膜材料中蛋黄卵磷脂(EPC)与胆固醇(CH)的质量比(A),蛋黄卵磷脂(EPC)与茶多酚(TP)的质量比(B),水油相的体积比(C)对影响茶多酚柔性脂质体包封率的影响,对结果进行二次多项式拟合,并根据模型优化处方,得到最优结果并进行验证。结果:确定最佳工艺条件为:卵磷脂与胆固醇比例为3.82:1,卵磷脂与茶多酚比例为2.68:1,油水相体积比为4.34:1,优化得到实际最优处方包封率为57.37%,实测值与理论值无显著性差异。结论:响应平面法用于茶多酚柔性脂质体处方的优化筛选是可行的,具有很好的预测性,此处方工艺稳定,重现性好,能制得较高包封率的茶多酚柔性脂质体。更多还原

【Abstract】 Objective: To optimize and prepare tea polyphenols-loaded flexible liposomes by Box-Behnken response surface methodology(RSM). Methods: The liposomes using EPC and CH as membranous material, sodium cholate hydrate as softener ware prepared by reverse phase evaporation technique and optimized thought a three factor and three-level Box-Behnken,with the EPC: CH(A), EPC: TP(B) and oil phase: water phase(C) as the independent variables, and the entrapment efficiency(Y) as the dependent variables.Second-order polynomial equations were fitted to the data,and the resulting equations were used to produce three dimensional response surface graphs,by which optimal experimental conditions were selected.Results:The optimal preparation conditions were determined as EPC/CH ratio 3.82:1,EPC/TP ratio 2.68:1,oil phase/ water phase ratio 4.34:1. Under these conditions, the evaluated entrapment efficiencyof the liposomes was 57.37%. There was no significant difference between the measured value with the theoretical value.Conclusions: The response surface method is applicable for the formulation and preparation conditions of optimization of teapolyphenols-loaded flexible liposomes, and the resulting optimal preparation technique is stable, feasible, and predictable.The liposomes with highly entrapment efficiencycan be prepared under optimal preparation conditions.更多还原