【摘要】 目的研究药典规定剂量附子水提组分单次给药对正常大鼠血压影响的"量-时-效"关系。方法制备1.35、1.08、0.81、0.54、0.27 g·kg-1(分别相当于药典规定附子用量15、12、9、6、3 g)5个不同剂量组的附子水提组分,给予大鼠灌胃上述剂量的附子水提组分,分别于药前,药后15、30、60、90、120 min,采用BP-100A全自动大小鼠无创血压测量系统观察不同剂量和时间下大鼠血压变化情况,并计算附子水提组分对大鼠正常血压影响的ED50值。结果附子水提组分对大鼠收缩压"量-时-效"关系研究显示:5个剂量组大鼠收缩压在药后30 min达峰,且水提组分1.35 g·kg-1剂量组升高幅度最大,药后120 min,5个剂量组的收缩压均基本回落到给药前水平;附子水提组分对大鼠舒张压"量-时-效"关系研究显示:水提组分对舒张压较给药前开始均有不同程度的升高;附子水提组分对大鼠正常血压影响的ED50值及其95%可信限为0.1474(2.3411~0.3482)g·kg-1。结论附子水提组分在药典规定剂量范围内使大鼠收缩压升高,并呈现一定的"量-时-效"关系,剂量愈高,升压作用愈大,作用时间愈长。更多还原

【Abstract】 Objective To study the influences of "dose-time-efficacy" relationship on normal rat blood pressure from a single administration of Aconite water extract in pharmacopoeia dose. Methods The 5 different Aconite water extract doses of 1.35, 1.08, 0.81, 0.54, 0.27 g·kg-1(which correspond in the pharmacopoeia Aconite dose 15, 12, 9, 6, 3 g) were prepared, and rats were given Aconite water extract by gavage respectively. Changes of rat blood pressure according to different time and dose were observed in 15, 30, 60, 90, 120 min respectively by BP-100 A automatic non-invasive blood pressure measurement system. And the ED50 of Aconite water extract was tested. Results The "dose-time-efficacy" relationship showed that: compared with normal group, the changes of blood pressure in rat were increasing marks from 15 min to 120 min. The systolic pressure in five dose groups hit the peak in 30 min and Aconite water extract high dose(1.35g·kg-1) is the largest. In 120 min, the systolic pressure in five dose groups were basically back to the front of the gavage. The diastolic blood pressure of rats by Aconite water extract was increasing marks from 15 min to 120 min to some extent. The ED50 and its 95% confidence limit of Aconite water extract were 0.1474(2.3411~0.3482) g·kg-1.Conclusion Aconite water extract in the pharmacopoeia dose range elevates rats systolic blood pressure, and there is a certain relationship of "dose-time-efficacy" : higher dose, greater pressor effects and longer action time.更多还原