【摘要】 目的:探讨藤梨根提取物对人胃癌BGC-823细胞凋亡的影响及其调控机制。方法:体外培养对数生长期胃癌BGC-823细胞,采用不同浓度的藤梨根提取物作用于人胃癌BGC-823细胞,流式细胞仪检测细胞凋亡,采用免疫组织化学方法检测Bax蛋白表达。结果:藤梨根作用于BGC-823,在8~32mg·mL-1时呈不同程度的促进凋亡;藤梨根提取物大、中、小剂量组对人胃癌BGC-823细胞中Bax表达水平上调。结论:藤梨根提取物对人胃癌BGC-823细胞的增殖具有明显抑制作用,在实验浓度范围内呈浓度依赖性促进细胞凋亡,这可能与激活Bax蛋白表达有关。更多还原

【Abstract】 Objective:To discuss the effects of Kiwi root extract on human gastric cancer BGC cell apoptosis and its regulatory mechanism.Methods:The gastric cancer BGC-823cells in logarithmic phase were cultivated in vitro.Kiwi root extract with different concentrations act on gastric cancer BGC-823cells.The cell apoptosis was detected by Flow cytometry instrument,and Bax protein expression was tested by immunohistochemical methods.Results:Kiwi root can act on BGC-823cells.Kiwi root in 8~32mgoml-1 can promote cell apoptosis in the different level.Kiwi root extract raised Bax expression levels of human gastric cancer BGC-823cells in large dose group,medium dose group or small dose group.Conclusion:Kiwi root extract has obvious inhibitory effect on human gastric cancer BGC-823cells proliferation.Kiwi root extract in the experimental concentration range can promote cell apoptosis in concentration dependence,which may be related to activation of Bax protein expression.更多还原