【摘要】 目的探讨MicroRNA-126(miR-126)在人结肠癌细胞中的表达以及对结肠癌细胞生物学行为的影响。方法应用荧光PCR定量检测60例结肠癌患者的结肠癌组织以及癌旁组织中miR-126的表达,共60对,再测定5株结肠癌细胞(SW480、HT29、HCT116、LOVO、SW620)中的表达情况;CCK-8法、划痕实验和Transwell实验检测细胞的增殖、迁移和侵袭能力,并进一步通过体外实验研究miR-126对结肠癌细胞生物学行为的影响。结果与癌旁组织细胞比较,miR-126在结肠癌细胞中表达下降(95.0%vs.55.0%,P<0.05),发生转移者与未发生转移者比较,差异有统计学意义(25.0%vs.75.0%,P<0.05)。miR-126可以抑制SW480细胞的生长及其侵袭转移,有基质胶Tran—swell实验中,转染组及空白对照组细胞迁移数分别为(10.0±4.3)个和(258.0±30.6)个;无基质胶的Transwell实验中,分别为(84.0±13.2)个和(335.0±27.3)个,差异均有统计学意义(P<0.05)。miR-126高表达可以增强癌细胞对奥沙利铂的敏感性。结论miR-126可明显抑制结肠癌细胞的发生发展,影响多种结肠癌细胞株生物学行为。更多还原

【Abstract】 Objective To investigate the expression of MicroRNA-126(miR-126) in human colon cancer cells and its effect on biological behavior of colon cancer cells.Methods Sixty patients with colon cancer were enrolled in the study.Fluorescence PCR was used to detect the expression of miR-126 in the cancerous tissues and in the tissues adjacent to carcinoma.The expression of miR-126 in the cancerous tissues paired up with the expression of miR-126 in the tissues adjacent to carcinoma,and there were 60 pairs in total.The expression of miR-126 in 5 colon cancer cell lines(SW480,HT29,HCT116,LOVO,SW620) was also detected.The proliferation,migration and invasion ability of the cells were measured using CCK 8 assay,scratch experiments and Transwell assay,respectively.The effect of miR-126 on the biological behaviors of human colon cancer cells was further studied through the experiments in vitro.Results Compared with adjacent normal tissue cells,miR-126 was decreased(95.0%vs.55.0%,P<0.05) in colon cancer cells,and were obviously when cancer metastasis or invasion is particularly evident,when compared with those without metasis,there were significant difference(25.0%vs.75.0%,P<0.05).Studies have shown that miR-126 can inhibit the growth of SW480 cells and their invasion and metastasis,Transwell assay with matrigel showed the migration cell number in transfection group and blank control group was(10.0±4.3)and(258.0±30.6),while Transwell assay without matrigel showed the migration cell number was(84.0±13.2) and(335.0±27.3)respectively,there were significant differences(P<0.05).miR-126 expression can enhance the sensitivity of cancer cells to oxaliplatin.Conclusion miR-126 can inhibit the development of colon cancer cells,the impact of a variety of strains of the biological behavior of colon cancer cells.更多还原