【摘要】 目的:研究新合成产物乙酰半胱氨酸镁盐-甘草酸环状样二聚体(acetylcysteine magnesiumglycyrrhizin fine antipode dimers,GFA-NAC·Mg)治疗大鼠肝纤维化的效果。方法:25%四氯化碳(CCl4)制备大鼠肝纤维化模型,GFA-NAC·Mg按25、50、100mg/kg剂量每日分别腹腔注射进行肝纤维化治疗,给药8周后处死大鼠,用肝纤维化网格染色观察肝纤维化情况,ELISA法测定血清羟脯氨酸(Hyp)和肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、转化生长因子-β1(TGF-β1)、血小板衍生生长因子(PDGF)。结果:大鼠肝组织病理结果显示其肝纤维化处于Ⅲ-Ⅳ期;与模型组比较,GFA-NAC·Mg治疗后血清Hyp明显降低;肝组织抗氧化指标SOD显著升高,MDA明显下降;肝纤维化指标TGF-β1、PDGF在组织含量下降;GFA-NAC·Mg各组网格染色显示肝纤维化好转。结论:GFA-NAC·Mg对肝纤维化大鼠有较好实验治疗作用,可能通过抑制PDGF和TGF-β1的表达而实现。更多还原

【Abstract】 AIM:To investigate efficacy of GFA-NAC· Mg on rat liver fibrosis.METHODS:Preparation of liver fibrosis model in rats with 25% carbontetrachloride(CCl4)by subcutaneous injection,GFA-NAC·Mg intraperitoneal injected treating liver fibrosis with 25,50and100 mg/kg.All the rats were killed after 8weeks of administration with grid staining and the levels of Hyp in serum were detected with ELISA assay,the contents of the MDA,SOD,TGF-β1,PDGF for liver fibrosis and liver tissue were tested.RESULTS:The liver tissue of fibrosis showed stageⅢ-Ⅳ.Compared with the model group,the serum of Hyp significantly was reduced after GFA-NAC·Mg treatment.The antioxidant index SOD significantly was increased in liver tissue while MDA was decreased.Liver fibrosis markers TGF-β1and PDGF content were decreased.Liver fibrosis was improved after GFA-NAC· Mg treatment in each groups by grid staining.CONCLUSION:GFA-NAC· Mg has a better therapeutic effect on rat liver fibrosis by inhibiting the expression of PDGF and TGF-β1.decreased.Liver fibrosis was improved after GFA-NAC· Mg treatment in each groups by grid staining.CONCLUSION:GFA-NAC· Mg has a better therapeutic effect on rat liver fibrosis by inhibiting the expression of PDGF and TGF-β1.更多还原