【摘要】 目的:采用高效液相色谱法,测定甘肃不同产区红芪中毛蕊异黄酮和芒柄花素含量,对产区与指标性成分的相关性进行研究。方法:色谱柱为TC-C18(2)柱(250 mm×4.6 mm,5μm),TC-C18保护柱(10 mm×4.6 mm,5μm),流动相为乙腈-0.01%磷酸水溶液(梯度洗脱),流速为1.0 m L·min-1,检测波长为248 nm,柱温为30℃,进样量为10μL。结果:经系统聚类分析方法分析,采自陇南市和定西市的16批样品可分为三大类,其中采自陇南市武都区、宕昌县的9个产地的红芪中毛蕊异黄酮和芒柄花素的含量较高,芒柄花素含量在111.72~424.92μg·g-1,毛蕊异黄酮含量在17.20~34.34μg·g-1;而采自定西市岷县、陇西县和宕昌县部分乡镇的红芪中毛蕊异黄酮和芒柄花素的含量较低,芒柄花素含量在74.35~182.88μg·g-1,毛蕊异黄酮含量在2.27~13.56μg·g-1。结论:不同产区红芪药材中指标性成分毛蕊异黄酮和芒柄花素含量存在一定的差异。武都、宕昌产红芪质量优于岷县、陇西等地所产红芪药材,这与武都、宕昌为红芪道地产区一致,可为红芪GAP种植基地的选址提供参考依据。更多还原

【Abstract】 Objective: To determine the contents of calycosin and formononetin in Hedysari Radix from different habitats by HPLC and explore the correlation between production areas and mark components. Methods: The sample was separated on TC-C18( 2)( 250 mm × 4. 6 mm,5 μm) column. The mobile phase consisted of acetonitrile and 0. 01% phosphoric acid solution( gradient elution) at a flow rate of1. 0 m L·min- 1. The UV detection wave length was set at 248 nm. The column temperature was set at 30 ℃.The injection volume was set at 10 μL. Results: The 16 samples from different habitats were divided into 3categories according to the result of hierarchical cluster analysis. The content of formononetin in Hedysari Radix from Wudu and Tanchang was between 111. 72 ~ 424. 92 μg·g- 1,and calycosin between 17. 20 ~34. 34 μg·g- 1; The content of formononetin in Hedysari Radix from Minxian and Longxi was between74. 35 ~ 182. 88 μg·g- 1,and calycosin between 2. 27 ~ 13. 56 μg·g- 1. Conclusion: The contents of calycosin and formononetin in Hedysari Radix from different habitats marked difference. It was found that those Hedysari Radix produced in Wudu and Tanchang are higher quality than those in Minxian and Longxi,which was consistent with the idea that Wudu and Tanchang were the genuine producing area of Hedysari Radix,and it can provide reference for establishing GAP base of Hedysari Radix.更多还原