【摘要】 目的研究食管癌组织中微小RNA(miRNA)let-7e的表达水平。方法以U6小核RNA为内参,应用RQ-PCR法检测40例食管癌组织及癌旁组织中miRNA let-7e的表达水平,分析其表达水平与临床病理参数的关系。结果 miRNA let-7e在食管癌组织的表达水平高于癌旁组织(10.54±5.50)倍(P<0.01);其与患者性别和肿瘤组织学类型、淋巴转移、分化程度及分期无明显相关。结论 miRNA let-7e在食道癌组织中表达升高,可能作为食道癌分子生物学的诊断标记。更多还原

【Abstract】 Objective To study the expression of microRNA(miRNA)let-7ein human esophageal cancer tissues.Methods U6 small nuclear RNA was taken as an internal marker and the expression level of miRNA let-7ein human esophageal cancer tissues and tumor-adjacent normal tissues in 40 patients with esophageal cancer were detected by RQ-PCR.The correlation between expression level of miRNA let-7eand clinical pathological parameters was analyzed.Results The expression level of miRNA let-7ein esophageal cancer tissues was(10.54±5.50)times higher than that in tumor-adjacent normal tissues(P<0.01),which was not significantly related to sex,histologic characteristic,lymph metastatic,differentiation and stage of the tumors.Conclusion The expression of miRNA let-7eis increased in esophageal cancer tissues,which may be taken as a molecular indicator for the diagnosis of esophageal cancer.更多还原