【摘要】 BGC823细胞瞬时转染绿色荧光蛋白(GFP)质粒2 d后,用FACSAria流式细胞仪分选GFP阳性和阴性BGC823细胞亚群,检测其分选纯度并培养分选后细胞,观察其无菌生长状态;商品化FITC直标抗体孵育SMMC7721细胞后,FACSAria流式细胞仪分选FITC阳性和阴性细胞亚群。将所有分选的细胞进行培养检测无菌状态,并观察细胞生长情况。FACSAria流式细胞仪分选得到的GFP阳性细胞群的分选纯度达99.6%,GFP阴性细胞群的分选纯度达98.8%,同时激光共聚焦荧光显微镜显示分选后的GFP阳性细胞明显富集;分选后的BGC823细胞和SMMC7721细胞常规培养,细胞均无污染且生长状态良好。更多还原

【Abstract】 BGC823 cells were transiently transfected with green fluorescent protein(GFP) plasmids.Two days later,GFP positive and negative cells were sorted by FACSAria.Separated cells were tested by FACSAria for purity analysis and also cultured for sterile analysis.SMMC7721 cells were incubated with FITC-labeled commercial antibody and then sorted on FACSAria for FITC positive and negative cells.All separated cells were cultured to sterile analysis.The purity for GFP positive and negative subpopulation sorted form GFP transfected BGC823 cells were99.6% and 98.8% respectively.Detected by laser scanning confocal microscopy,the separated GFP positive cells were significantly enriched after sorting compared to parent cells.Sorted BGC823 cells and SMMC7721 cells were cultured conventionally and cells were all free from contamination with a good growth.FACSAria flow cytometry is suitable to preparation of sterile cell subpopulation with high purity both from fluorescence plasmids transfected cells and fluorescence antibody labeled cells.更多还原