【摘要】 目的观察重组人内抑素(rhEndostatin)对兔耳瘢痕增生的抑制作用,探讨其部分作用机制。方法建立兔耳增生性瘢痕(HS)模型,瘢痕块内局部注射rhEndostatin(1.25、2.5、5 g/L),观察瘢痕大体形态及组织学变化,免疫组织化学检测瘢痕组织Ⅰ、Ⅲ型胶原表达,电镜观察瘢痕组织成纤维细胞凋亡。结果与模型组和生理盐水组比较,2.5 g/L和5 g/L rhEndostatin组瘢痕平坦,面积减小,质地变软,瘢痕增生指数降低(P<0.05);微血管密度降低(P<0.05);Ⅰ、Ⅲ型胶原表达水平降低(P<0.05);成纤维细胞凋亡。结论rhEndostatin可抑制HS形成,该作用可能与rhEndostatin抑制血管生成,进而促进增生性瘢痕成纤维细胞(HSFs)凋亡,减少胶原的产生与沉积有关。更多还原

【Abstract】 Objective To investigate the therapeutic effects of recombinant human endostatin(rhEndostatin) on hypertrophic scar(HS) and explore the possible mechanism of action involved.Methods A rabbit ear model with hypertrophic scars was established.Recombinant human endostatin injections were applied to the scars of the experimental group topically in different concentrations(1.25,2.5,5 g /L).The changes of macroscopic characteristics of scars were examined.Light microscopy and transmission electron microscopy were used for histomorphometric analysis.The levels of collagens Ⅰ and Ⅲ were detected by immunohistochemistry.Results Compared with physiological saline group and model group,group treated with rhEndostatin 2.5 g /L and 5.0 g /L showed significant reduction in height,volume and hardness,it resulted in a statistically significant reduction in the scar elevation index,the microvessel density and the expression of type Ⅰ and Ⅲ collagens(P < 0.05).An observation under transmission electron microscopy revealed that some fibroblasts nucleus ruptured and disintegrated and apoptotic body were observed in rhEndostatin 5 g /L group,but seldom found in triamcinolone group.Conclusion The findings of this study suggest that rhEndostatin has a markedly inhibitive effect on the formation of hypertrophic scar.It suppresses angiogenesis,and subsequently promotes HSFs apoptosis and reduces the overproduction and excessive deposition of collagen may account for this result.更多还原