【摘要】 目的探讨冠状动脉粥样硬化性心脏病(冠心病)患者血清内脂素与磷酸化c-jun、CD40L及组织因子(TF)的关系。方法选择不稳定型心绞痛患者65例(UAP组)、稳定型心绞痛(SAP)患者75例、对照组60例,采用ELISA方法测定血清内脂素、磷酸化c-jun、TF及组织因子途径抑制物(TFPI)浓度水平,采用流式细胞技术测定CD40L平均荧光强度。结果 UAP组患者血清内脂素、磷酸化c-jun、CD40L、TF、TFPI水平及TF/TFPI值高于SAP组及对照组(P<0.05或P<0.01);SAP组上述指标均高于对照组(P<0.05或P<0.01)。线性相关分析显示冠心病患者血清内脂素水平与磷酸化c-jun、CD40L、TF、TFPI水平及TF/TFPI值呈正相关(P均<0.01)。多重线性逐步回归分析表明冠心病患者血清内脂素水平与磷酸化c-jun、CD40L、TF水平呈独立正相关(P均<0.01)。结论血清内脂素水平升高可能促进炎症标志物磷酸化c-jun、CD40L及组织因子生成,影响冠心病发生、发展及预后。更多还原

【Abstract】 Objective To investigate the relationship between serum visfatin and phosphorylated c-Jun,CD40 L and tissue factors( TF) in patients with coronary heart disease. Methods In total,200 subjects were enrolled in this study and divided into the control group( n = 60),unstable( n = 65) and stable angina groups( n = 75). Serum visfatin,phosphorylated c-Jun,TF and tissue factor pathway inhibitor( TFPI)were measured using ELLSA,while the percentage of positive CD40 L was quantified by flow cytometry. Results The serum levels of visfatin,phosphorylated c-Jun,CD40 L,TF,TFPI and the ratio of TF /TFPI in the stable angina group were significantly higher compared with those in the control group( P < 0. 05 or P < 0. 01).All these parameters in patients with unstable angina were significantly higher than their counterparts with stable angina( P < 0. 05 or P < 0. 01). Linear correlation analysis revealed that serum visfatin was positively correlated with phosphorylated c-Jun,CD40 L,TF,TFPI and TF /TFPI( all P < 0. 01). Multiple linear stepwise regression analysis demonstrated that serum visfatin was independently and positively associated with phosphorylated c-Jun,CD40 L and TF( all P < 0. 01). Conclusion Hypervisfatinemia probably influences the incidence,development and prognosis of coronary heart disease by elevating the levels of phosphorylated c-Jun,CD40 L and TF.更多还原