【摘要】 目的探讨BAG-1、Bcl-2在子宫内膜癌中的表达情况。方法采用免疫组织化学链霉菌抗生素蛋白-过氧化物酶连接法检测正常子宫内膜组、子宫内膜增生过长组、子宫内膜不典型增生组和子宫内膜癌组组织中BAG-1和Bcl-2蛋白的表达情况。结果 BAG-1在子宫内膜癌中呈高表达,其阳性率为64.0%,明显高于正常子宫内膜组、子宫内膜增生过长组和子宫内膜不典型增生组(9.5%、26.7%和30.8%),差异均有统计学意义(P<0.05)。Bcl-2在子宫内膜癌中表达阳性率(46.0%)明显低于子宫内膜增生过长组(73.3%),差异有统计学意义(P<0.05)。结论 BAG-1在子宫内膜癌的发生、发展及转移中起重要作用。BAG-1表达越高,子宫内膜癌的组织学分化程度越低,临床分期越晚,癌组织越容易浸润,提示其恶性程度越高,患者预后不良。Bcl-2的表达参与子宫内膜癌的发生、发展过程并且与肌层浸润相关,但是与组织学分化无相关性,因此缺乏预测预后的证据。更多还原

【Abstract】 Objective To observe the expression of BAG-1 and Bcl-2 gene in endometrial adenocarcinoma. Methods The immunohistochemical streptavidin-perosidase(SP) method was applied to detect the expression of BAG-1 protein and Bcl-2 protein in normal endometrium group,endometrial hyperplasia group,endometrial atypical hyperplasia(EAH) group and endometrial adeno carcinoma. Results Over-expression of BAG-1 was detected in endometrial adenocarcinoma with the positive rate of 64.0%,which was apparently higher than that in nomal endometrium group,endometrial hyperplasia group and EAH group with positive rate of 9.5%,26.7% and 30.8% respectively,and the difference had statistical significance(P<0.05). The positive rate of the expression of Bcl-2 in endometrial carcinoma group was significantly lower than that in endometrial hyperplasia group(46.0% vs. 73.3%),and the difference was statistically significant(P<0.05). Conclusion The BAG-1 plays an important role in the occur rence,development and metastasis of endometrial cancer. The higher expression of BAG-1 is,the lower histological differentiation degree of endometrial cancer becomes,the later clinical stage is,the more easily cancer tissue infiltrates,the higher degree of malignancy is and the poorer prognosis of patients is. The expression of Bcl-2 involves in the development of endometrial adenocarcinoma,and it is related to myometrial invasion,but without correlation with histological differential,therefore,it lacks the evidence to predict prognosis.更多还原