【摘要】 本研究旨在了解临床常见的EDTA依赖的假性血小板减少的发生率及其解决方法。对2010年1月-2013年5月间住院的71 535例,在血常规检测时有血小板减少的1326例患者进行了对比分析,并对其中EDTA-K2抗凝剂导致的假性血小板减少病例87例改用枸橼酸钠抗凝剂再次分析检测,同时涂片瑞-姬氏染色油镜下观察血小板形成分布情况。结果表明:87例EDTA-K2抗凝剂血小板数值为(56±27)×109/L,换用枸橼酸钠抗凝剂在同一仪器上检测,其血小板数值为(185±39)×109/L,两者结果用配对t检验分析,统计学上结果有显著性差异(t=1.83,P<0.01)。EDTA-K2抗凝剂导致的假性血小板减少,其发生率为0.12%,占血小板减少总数的6.56%。结论:EDTA-K2抗凝剂导致的假性血小板减少发生率为0.12%,极易误诊。对血小板计数<100×109/L的标本应进行涂片复查。发现有血小板聚集的情况应改用枸橼酸钠抗凝剂进行再次检测,以防发生误诊误治。更多还原

【Abstract】 The purpose of this study was to investigate the incidence of clinically common EDTA-dependent pseudothrombocytopenia( EDTA-PTCP) and methols for treating this diseese. A total of 1326 cases of thrombocytopenia found at blood routine examination were amalyzed anong 71 535 patients hospitalized in our hospital from January 2010 to May2013,and 87 cases of PTCP caused EDTA-K anticoagulant were analyzed again by using sodium citrate auticoagulant,at the same time the platelet formation distribution was observed by microscopy of smear with Wright-Giemsa staining. The results showed that the platelet count detected by using EDTA-K anticoagulant in 87 cases was( 56 ± 27) × 109/L,while the platelet count detected by using sodium citrate was( 185 ± 39) × 109/L( t = 1. 83,P < 0. 01). The pseudothrombocytopenia incidence cansed by EDTA-K was 0. 12%,it was 6. 56% for the total number of thrombocytopenia. It is concluded that the incidence of PTCP cansed by EDTA-K is 0. 12%,the PTCP is easily misdiagnosed. Therefore,the specimens of platelet count < 100 109/L should be tested again. When the platelet aggregation is found,the specimens should be examined again by using sodium citrate in order to avoid misdiagnosis.更多还原