【摘要】 目的建立高效液相色谱法同时测定参茸延龄片中补骨脂素、异补骨脂素、淫羊藿苷和宝藿苷Ⅰ含量的方法。方法采用Hypersil C18色谱柱(4.6 mm×250 mm,5μm);流速:1.1 mL/min;流动相A为乙腈-甲醇(2∶1),流动相B为0.1%冰醋酸溶液,梯度洗脱;检测波长分别为246 nm(补骨脂素和异补骨脂素)、270 nm(淫羊藿苷和宝藿苷Ⅰ)。结果补骨脂素、异补骨脂素、淫羊藿苷和宝藿苷Ⅰ分别在0.033 2~0.664 0μg(r=0.999 8)、0.022 6~0.452 0μg(r=0.999 1)、0.029 4~0.588 0μg(r=0.999 5)、0.024 2~0.484 0μg(r=0.999 4)范围内进样量与峰面积呈良好的线性关系,平均加样回收率分别为97.48%、98.44%、99.13%、98.71%,RSD分别为0.92%、1.39%、1.17%、1.21%。结论本方法简便、准确、灵敏、重复性好,可作为参茸延龄片中补骨脂素、异补骨脂素、淫羊藿苷和宝藿苷Ⅰ的含量控制方法。更多还原

【Abstract】 Objective To develop an HPLC method for determination of the contents of psoralen, isopsoralen, icariin and baohuoside Ⅰ in Shengrong Yanling Pill. Methods Hypersil C18 column was used as the chromatographic column, with gradient elution, the flow rate of 1.1 mL/min, the mobile phase A of acetonitrile-methanol(2∶1), the mobile phase B of 0.1% glacial acetic acid solution, and detection wavelength of 246 nm(for psoralen and isopsoralen) and 270 nm(for icariin and baohuoside Ⅰ). Results There was a good linear relationship between the injection volume and peak area valued when the volume of psoralen, isopsoralen, icariin and baohuoside Ⅰwere within the range of 0.033 2-0.664 0 μg(r =0.999 8), 0.022 6-0.452 0 μg(r =0.999 1), 0.029 4-0.588 0 μg(r =0.999 5), and 0.024 2-0.484 0 μg(r =0.999 4), respectively. The average recovery rates were 97.48%(RSD=0.92%), 98.44%(RSD=1.39%), 99.13%(RSD=1.17%) and 98.71%(RSD=1.21%), respectively. Conclusion The method was convenient, accurate, sensitive and repeatable, and could be used in the content control method of psoralen, isopsoralen, icariin and baohuoside Ⅰ in Shengrong Yanling Pill.更多还原