【摘要】 目的探讨肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)在慢性乙型肝炎(CHB)和原发性肝癌(PHC)患者血清及肝组织中的表达水平。方法应用酶联免疫吸附测定法检测45例CHB患者(CHB组)、45例PHC患者(PHC组)及45例健康人(对照组)血清中TNF-α、IL-1β水平,采用免疫组织化学方法检测CHB和PHC患者肝组织中TNF-α、IL-1β蛋白的表达,并对3组结果进行比较。结果 CHB组患者血清TNF-α和IL-1β水平高于对照组(P=0.000),PHC组患者血清TNF-α和IL-1β水平显著高于CHB组(P=0.000)。PHC组织中TNF-α和IL-1β蛋白阳性表达显著高于CHB组织(P=0.000)。结论 TNF-α和IL-1β是重要的促炎性细胞因子,在CHB和PHC患者的肝损伤过程中发挥重要作用,可作为临床生物治疗的靶点。更多还原

【Abstract】 Objective To study the expression of tumor necrosis factor-α( TNF-α) and interleukin-1β( IL-1β) in the serum and hepatic tissues of patients with chronic hepatitis B( CHB) or primary hepatic cancer( PHC). Methods The levels of serum TNF-α and IL-1β in 45 patients with CHB( CHB group),45 patients with PHC( PHC group) and 45 healthy people( control group) were detected by enzyme-linked immunosorbent assay. The expression of TNF-α and IL-1β in CHB and PHC tissues were detected by immunohistochemistry method. The results were compared among the three groups. Results The levels of serum TNF-α and IL-1β in CHB group were significantly higher than those in control group( P = 0. 000). The levels of serum TNF-α and IL-1β in PHC group were significantly higher than those in CHB group( P = 0. 000). The positive expression of TNF-α and IL-1β in PHC tissues was significantly higher than that in CHB tissues( P = 0. 000). Conclusion TNF-αand IL-1β as the important proinflammatory cytokines,play an important role in the process of liver injury in patients with CHB or PHC,and can be used as clinical biological treatment targets.更多还原