【摘要】 目的探讨人脐带间充质干细胞(hUCMSCs)裂解液在体外对食管癌EC9706细胞增殖、迁移的抑制作用。方法用组织块培养法体外培养hUCMSCs,流式细胞仪检测细胞表型;收集hUCMSCs,并用反复冻融法制备hUCMSCs裂解液;不同浓度hUCMSCs裂解液作用于食管癌EC9706细胞一定时间,采用四甲基偶氮唑盐(MTT)法检测细胞增殖状况,倒置显微镜观察细胞形态。通过Tranwell迁移实验观察hUCMSCs裂解液对食管癌EC9706细胞迁移能力的影响。结果人脐带组织块培养7~12 d后可见贴壁的成纤维样细胞,流式细胞仪分析显示第5代细胞均表达CD29、CD90和CD166,不表达CD34、CD45和人类白细胞抗原-DR。MTT法显示加入hUCMSCs数等于或大于食管癌细胞数的裂解液时,与对照组比较明显抑制食管癌细胞的生长(P<0.05),且实验组各组抑制食管癌细胞增殖能力差异无统计学意义(P>0.05)。Tranwell迁移实验显示,实验组EC9706细胞的迁移较对照组明显受到抑制(P<0.05)。结论 hUCMSCs裂解液对食管癌细胞EC9706的增殖和迁移均有抑制作用。更多还原

【Abstract】 Objective To investigate the suppressive effect of human umbilical cord mesenchymal stem cells( hUCMSCs) lysate on proliferation and migration of esophageal cancer EC9706 cells in vitro. Methods hUCMSCs was cultured by using tissue culture in vitro and cell phenotype analysis was performed by using flow cytometry. hUCMSCs were collected and hUCMSCs lysate was prepared through repeated freezing and thawing. Different concentration of lysate were added to esophageal cancer EC9706 cells culturing for a period of time,cell proliferation was evaluated with 3-( 4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide( MTT) assay and cell morphology was observed by using inverted microscope. Tranwell experiment was exerted to observe the effect of hUCMSCs lysate on the migration of EC9706 cell. Results When hUCMSCs was cultured for 7- 12 days,adherent and fibroblast-like cells were visible. Flow cytometry analysis revealed that CD29,CD90 and CD166 were highly expressed on the plasma membrane of the 5thgeneration cells,but negative for CD34,CD45 and human leucocyte antigen-DR. MTT assay showed that lysate obviously inhibited the growth of esophageal cancer cells compared with the control group when the adding number of hUCMSCs was equal to or greater than the number of esophageal cancer cells( P < 0. 05),and the inhibition of cell proliferation of each experimental group in esophageal cancer had no significant difference( P >0. 05). Tranwell experiment showed that the experimental group EC9706 cell migration was significantly inhibited compared with the control group( P < 0. 05). Conclusion hUCMSCs lysate has an inhibition effect on both proliferation and migration of esophageal cancer EC9706 cells in vitro.更多还原