【摘要】 目的 探讨PPARs激动剂吡格列酮对RAW264.7巨噬细胞源性泡沫细胞内胆固醇含量的影响。方法 首先用50 mg/L氧化型低密度脂蛋白(ox-LDL)将体外培养的RAW264.7巨噬细胞诱导分化为泡沫细胞,然后进行油红0染色,并在光镜下鉴定泡沫细胞形态及变化;再以不同浓度吡格列酮(0μmol/L5μmol/L、10μmol/L、20μmol/L、30μmol/L)作用泡沫细胞24 h,以30μmol/L的吡格列酮作用0 h、6 h、12h、24 h、48 h;最后酶法检测泡沫细胞内胆固醇的含量。结果 ①50 mg/Lox-LDL诱导巨噬细胞48 h后分化为泡沫细胞。②与对照组相比,不同浓度吡格列酮作用后泡沫细胞内胆固醇含量均减少,差异有统计学意义(P<0.01),且呈剂量依赖性。③与对照组相比,30μmol/L的吡格列酮作用6 h、12 h、24 h、48 h后,泡沫细胞内胆同醇含量均减少,差异有统计学意义(P<0.01),且呈时间依赖性。结论 PPARs激动剂吡格列酮能够减少泡沫细胞内胆固醇含量,且呈剂量和时间依赖性。更多还原

【Abstract】 Objective To observe the effects of PPARs agonist Pioglitazone on cholesterol levels in RAW264.7 macrophage-derived foam cell.Methods Firstly,with 50 mg/L oxidized low density lipoprotein(oxLDL) induce the mouse RAW264.7 cells into foam cells.Secondly,using red oil 0 staining technique and transmission light microscope was applied to appraise the morphology and change of foam cells.Thirdly,Pioglitazone of different concentrations(0 μmol/L,5 μmol/L,10 μmol/L,20 μmol/L,30 μmol/L) were incubated with RAW264.7 macrophage-derived foam cell for 24 h and 20 μmol/L pioglitazone were incubated with RAW264.7 macrophage-derived foam cell for 6 h,12 h,24 h,48 h.Lastly,the cholesterol content of foam cells was measured by enzyme assay.Results(1)The mouse RAW264.7 cells could be differentiated into macrophages after 48 h induction by 50 mg/L oxidized low density lipoprotein(ox-LDL).(2)Compared with the control group,treatment of RAW264.7 macrophage-derived foam cell with 5 μmol/L,10 μmol/L,20 μmol/L,30 μmol/L pioglitazone for 24 h,resulted in an decrease in the cholesterol content of foam cells,differences are statistically significant(P<0.01).(3)Compared with the control group,treatment of RAW264.7 macrophage-derived foam cell with 20 μmol/L pioglitazone for 6 h,12 h,24 h,48 h,resulted in a decrease in the cholesterol content of foam cells,differences were statistically significant(P<0.01).Conclusion PPARs agonist pioglitazone reduces cholesterol levels in foam cells in dose and time dependent.更多还原