【摘要】 目的 探讨制作兔心肌缺血再灌注模型的改进方法。方法 20只新西兰大白兔,“六线六结”改良法开胸后,随机以“双线双结”法结扎兔冠状动脉左前降支(常规组,10只)和左室支(观察组,10只)30 min,松结实施再灌注2 h后,取心测梗死面积。术前及再灌注2 h后,检测血肌钙蛋白I。结果 两组制模成功率均为70%;两组手术后肌钙蛋白I较术前均明显升高,左室支结扎组升高更明显;观察组造成的心肌梗死面积明显大于常规组(P<0.05),且变异小。结论改良法可有效地提高兔心肌缺血再灌注模型构建的成功率。更多还原

【Abstract】 Objective The research is aimed at improving methods in making of heart ischemic/reperfusion model of rabbit.Methods 20 New Zealand rabbits were randomly allocated to 2 groups(n=10) followed the procedure of "6 sutures and 6 knots" method to open the chest.Left anterior descending artery(LAD,control group) or left ventricular artery(LVA,study group) were occluded for 30 min using the "2 sutures and 2 knots" method,respectively.The hearts were enucleated after 120 min reperfusion,and myocardial infarct size were determined.Bloods were drawn from femoral vein to evaluate cardiac troponin I(cTnI) values before operation and120 min after reperfusion in both groups.Results Incidence of pneumothorax and hemorrhea were significantly reduced during operation in this research,and achievement ratios were 70%in the 2 groups.There was an obvious elevation of cTnI in blood after operation(control group:0.37±0.12 vs 2.31±1.59,P<0.05.Study group:0.32±0.14 vs 3.49±0.74,P<0.01).The infarct size in LVA occlusion group was bigger than that of LAD group,with less variation furthermore(control group:21.51±7.87%.Study group:28.95±6.59%,P<0.05).Conclusion The new methods would improve achievement ratio of establishing ischemic/reperfusion model in rabbit’s heart.更多还原