【摘要】 目的 研究低HDL-C患者外周血白细胞ABCA1和ABCG1 mRNA的表达状态。方法 采用实时荧光定量PCR方法,测定外周血白细胞ABCA1和ABCG1 mRNA表达水平。结果 低HDL-C患者ABCA1表达水平较对照组显著降低(0.23±0.09比1.18±0.49,P=0.042),ABCG1 mRNA表达差异无统计学意义(3.09±1.08比3.94±1.48,P=0.355);低HDL-C者高敏CRP(hs-CRP)水平明显高于对照组(2.34±1.68比1.15±0.48,P=0.008);血清hsCRP水平与ABCA1 mRNA表达呈负相关(r=-0.330,P=0.043),与血清HDL-C水平无相关性(r=0.19,P=0.254)。多因素线性回归分析表明,ABCA1 mRNA表达水平与血清hs-CRP水平呈边缘性相关(β=-0.330,P=0.079)。结论 低HDL-C患者外周血ABCA1 mRNA表达水平降低,炎症因素或许是其机制之一。这可能是低HDL-C者心血管病风险增高的一个因素。更多还原

【Abstract】 Objective To investigate ATP binding cassette transporters(ABCA1 and ABCG1) gene expression in peripheral blood leukocytes from subjects with low plasma HDL cholesterol levels.Methods Thirtyeight cases were enrolled,peripheral blood leukocytes ABCA1 and ABCG1 mRNA expression were measured by real-time quantitative PCR.Results ABCA1 mRNA expression in subjects with low plasma HDL cholesterol levels was obviously less than that in controls(0.23±0.09 vs 1.18±0.49,P=0.042),there was not different between two groups for ABCG1 mRNA expression(3.09± 1.08 vs 3.94±1.48,P=0.355).The serum hscrp concentration in subjects with low plasma HDL cholesterol levels was statistically greater than that in controls(2.34 ±1.68 vs 1.15±0.48,P=0.008),negatively associated with ABCA1 mRNA expression(r=-0.330,P=0.043),and not related to plasma HDL-C concentration(r=0.19,P=0.254).Using multivariant linear regression analysis method.it was shown that ABCA1 mRNA expression was borderly associated with the serum hsCRP level(β=-0.330,P=0.079).Conclusion ABCA1 mRNA expression in the peripheral blood leukocytes from subjects with low plasma HDL cholesterol levek was reduced,inflammation maybe a cause,and it is one probably factor to result in higher cardiovascular disease risk for these patients.更多还原