【摘要】 以甘蓝型黄籽油菜高硫苷GH06和甘蓝型双低油菜油研2号为亲本杂交,后代通过"一粒传法"连续自交6代构建重组自交系,利用HPLC法对亲本及重组自交系群体株系种子中硫苷组份进行测定,发现2-羟基-3-丁烯基硫苷和3-丁烯基硫苷是影响硫苷质量浓度的主导成分,利用本实验室已构建的遗传连锁图谱和复合区间作图法(CIM),分析种子中5种硫苷主要成分的QTL.结果共检测到11个QTL,分别位于8个不同的连锁群,其中3个2-羟基-3-丁烯基硫苷质量浓度的QTL,分别位于第10,14和24连锁群,单个QTL解释表型变异的5.54%~22.02%;3个3-丁烯基硫苷质量浓度的QTL,分别位于第3,10和24连锁群上,单个QTL解释表型变异的5.88%~10.33%;2个4-戊烯基硫苷质量浓度的QTL,分别位于第9和20连锁群上,单个QTL分别解释表型变异的12.04%和5.70%;3个苯乙基硫苷质量浓度的QTL,分别位于第9,15和21连锁群,单个QTL解释表型变异的6.29%~7.44%.解释表型变异大于10%的4个QTL;包括2个2-羟基-3-丁烯基硫苷质量浓度QTL,3-丁烯基硫苷质量浓度QTL和4-戊烯基硫苷质量浓度QTL各1个,它们分别解释16.32%,22.02%,10.33%和12.04%的表型变异.研究结果表明种子硫苷组份质量浓度遗传具有母本效应,且为多基因控制的数量性状,基因表达受环境影响较大,与这些QTL紧密相关的分子标记可以用于硫苷组份的分子标记辅助选择.更多还原

【Abstract】 A population of recombinant inbred lines(RILs)was derived from a cross between the female parent GH06 with higher glucosinolate contents and the male parent Youyan2 with lower erucic and glucsinolate content,and the progeny was selfed for 6successive generations by single seed propagation.The glucosinolate profiles were detected in the two parents and in the individuals of the population with HPLC.Five main glucosinolate profiles [Progoitrin(PRO),Gluconapin(NAP),4-hydroxyglucobrassicin(4OH),Glucobrassicanapin(GBN)and Gluconasturtin(NAS)]were identified in each of the RILs.Variation of glucosinolate component analysis showed PRO and NAP were the main glucosinolate profiles in the seed of B.napus.Using the genetic linkage map of B.napus constructed by our laboratory and the composite interval mapping(CIM)method,a total of 11 QTLs for the 5glucosinolate profiles were detected in8 different linkage groups,of which 3PRO-related loci were detected and mapped in Linkage groups N10,N14 and N24,respectively,explaining 5.54%-22.02% of the phenotypic variation;3NAP-associated QTLs were mapped in Linkage groups N3,N10 and N24,accounting for 5.88%-10.33% of the phenotypic variation;2 4OH-controlling QTLs were located in Linkage groups N9 and N20,which explained12.04%and 5.70%of the phenotypic variation,respectively;and 3QTLs involving in NAS accumulation were detected in Linkage groups N9,N15 and N21,respectively,explaining 6.29%-7.44%of the phenotypic variation.Four major QTLs explaining over 10% of the phenotypic variation were found,including2,1and 1for PRO,NAP and 4OH,i.e.qPRO-10-1,qPRO-24-3,qNAP-24-3and qGBN-9-1,which accounted for 16.32%,22.02%,10.33%and 12.04%of the phenotypic variation,respectively.These results showed that the inheritance of GS profiles was a quantitative trait under polygenic control,mainly influenced by maternal effects and environment.Molecular makers closely linked with these QTLs could be applied in marker-assisted selection of glucosinolate profiles in B.napus.更多还原