【摘要】 本研究选择生长速度不同的高邮鸭和金定鸭为试验材料,对不同品种鸭胚胎期和出雏早期肌肉中钙蛋白酶3(calpain3,CAPN3)基因的表达及其与肌纤维性状的关联性进行了研究。采用实时荧光定量PCR方法研究鸭13、17、21、25、27胚龄和出雏后7日龄胸肌和腿肌中CAPN3基因表达的发育性变化。结果发现,CAPN3mRNA在2个品种鸭早期肌肉发育中具有相似的表达模式,并表现出显著的时间特异性,而品种和性别效应并不显著。在胸肌中,呈现先下降后上升的趋势,25胚龄前表达量均较低,21胚龄最低,出雏前(27胚龄)至7日龄表达量显著上升。在腿肌中,呈现"波浪形"表达模式,在21胚龄表达量最高。腿肌CAPN3基因表达与肌纤维类型、直径、面积和密度之间呈现不同程度的线性相关,部分指标在金定鸭中达到了显著水平。初步推测,CAPN3基因与肌纤维类型和肌纤维的生长发育密切相关。更多还原

【Abstract】 In the present study,expression of CAPN3gene was quantified by RT-PCR in the breast muscle and leg muscle tissues on days 13(E13d),17(E17d),21(E21d),25(E25d),27(E27d)of embryonic development,as well as at 7days post-hatching(7dPH)in Gaoyou and Jinding ducks with different growth rates.Meanwhile,the association between expression level of CAPN3gene and myofiber traits was also analyzed.The results showed that the CAPN3mRNA expression profile had a similar trend in both breeds and showed an extremely significant specific expression at different development stages,breeds and gender had no effect on its expression.In breast muscle,the expression level of CAPN3mRNA was relatively low before E25dand the lowest point appeared on E21d,then increased significantly before hatching(E27d)and 7dPH.In leg muscle,the expression profile of CAPN3showed a"wave"trend,the expression peaking appeared on E21d.Meanwhile different linear correlations were found between CAPN3gene mRNA expression and myofiber types,diameter,cross-sectional area and density in duck.In Jinding ducks,CAPN3gene mRNA expression was significantly correlated with myofiber diameter,crosssectional area and density.These results suggest that CAPN3 may have potential functions in controlling muscle fiber phenotype and development during embryonic and early post-hatching development in ducks.更多还原