【摘要】 根据沙门菌属特异性基因hut及型特异性基因SdfⅠ、Spy、glgC和Spec序列,设计5对引物,建立沙门菌多重PCR方法,并用临床样品分离菌株对该方法进行检验。结果显示,该方法能特异性地鉴定肠炎沙门菌、鼠伤寒沙门菌、鸡白痢沙门菌和鸡伤寒沙门菌,4种细菌检测灵敏度均为3×103 CFU,染色体DNA检测灵敏度分别为162、202、214和178pg·μL-1。55份临床分离株检测的结果显示,与传统血清学方法相比,此多重PCR方法敏感性为100%,符合率最低为96.4%。该法能快速而准确地鉴定上述4种血清型的沙门菌。更多还原

【Abstract】 Based on the sequences of the hutgene specific for Salmonellaand the SdfⅠ,Spy,glgC,and Spec genes specific for Salmonellaserotypes,five pairs of primers were designed and synthesized.A multiplex PCR(mPCR)method was developed and uses for detection of clinical Salmonellaisolates.The results showed that the strains of S.enteritidis,S.typhimurium,S.pullorumand S.gallinarum were identified specifically by the mPCR,the sensitivities of mPCR for all bacteria were 3×103 CFU,and the sensitivities of mPCR for chromosomal DNA of S.enteritidis,S.typhimurium,S.pullorumand S.gallinarum were 162,202,214and 178pg·μL-1,respectively.Compared to the traditional serological assay,the sensitivities of the PCR for 55 Salmonellaclinical isolates were 100%,and the minimal coincidence rate of the mPCR assay were 96. 4%.The results indicated that the mPCR assay is a rapid,sensitive and specific method for identification of the four serotypes Salmonella.更多还原