【摘要】 为了研究分析新疆盐湖嗜盐古菌资源,从艾丁湖中分离筛选出一批嗜盐古菌,对其进行了形态特征,生理生化特性研究和分子鉴定。发现其中1株菌(D-20)的生理生化特性和产淀粉酶特性比较突出,经不同温度,不同pH,不同温度下的研究以及16SrDNA序列的系统发育学研究表明:该菌株为革兰氏染色阴性菌,最适生长温度为37℃,最适生长pH 8.0,最适盐浓度15%,能产过氧化氢酶、尿酶,淀粉酶,硝酸盐还原为阳性,不产H2S,对青霉素,氯四环素,红霉素,新霉素具有抗性,能利用赖氨酸,蛋氨酸,精氨酸,以果糖,蔗糖和核糖作为碳源。经分子鉴定该菌是Natrinema属的新成员,定名为Natrinema aidingensis D-20,Gene Bank登录号为AY298734。菌株保藏号为1.2657。更多还原

【Abstract】 In order to analysis ofhalophilic archaea from Xinjiang Saline Lake resources research,selected a group of halophilic archaea isolated from Aydingkol lake,its morphological characteristics,physiological and biochemical characteristics and molecular identification.Found that 1strains of bacteria(D-20)on physiological and biochemical characteristics and amylase producing conspicuous characteristics, with different temperature,different pH,studies show that growth under different temperature and 16S rDNA sequence of the system:the strain is gram negative bacteria,optimum growth temperature was 37 ℃,the optimum growth pH 8,the most salt concentration 15%,can produce catalase,urease,amylase, nitrate reduction is positive,do not produce H2S,penicillin,tetracycline,erythromycin,neomycin resistant,can make use of lysine,methionine,arginine,fructose,sucrose and ribose as carbon source.The strain D-20is a novel member of the Natrinema aidingensis of Bank,Gene accession number is AY298734.更多还原