【摘要】 目的建立湖南省健康成人外周静脉血T淋巴细胞亚群的参考范围。方法收集113名健康成人(18~60岁)静脉全血标本,用三色流式细胞术检测CD3+、CD4+及CD8+淋巴细胞绝对数值,百分率和CD4+/CD8+比值。结果CD3+、CD4+和CD8+淋巴细胞的绝对计数的中位数分别为1 422、736和546个/μl,CD4+、CD8+淋巴细胞的百分率的中位数为52.56%和39.98%,CD4+/CD8+比值的中位数为1.34。各参数值在不同年龄性别间分布差异无统计学意义(P>0.05)。结论本研究成功建立了湖南省健康成人T淋巴细胞亚群正常参考范围,可为本地区临床医生提供有用的参考依据。更多还原

【Abstract】 Objective The purpose of this reasech was to establish the reference ranges on peripheral blood T-lymphocyte subsets in healthy adults in Hunan. Methods One hundred and thirteen whole blood samples were collected from healthy adult from 18 to 60 years old. Absolute counts and percentage of T-lymphocyte subsets of blood samples were calculated by three-color flow cytometer.Results The median values of the T-lymphocyte subsets were 1 422 cells/μl for CD3+, 736 cells/μl for CD4+and 546 cells/μl for CD8+. The median values of the percentage of T-lymphocyte subsets were 52.56% for CD4+and 39.98% for CD8+, and the ratio was1.34 for CD4+/CD8+. No significant differences were observed for different age and gender(P>0.05). Conclusion The reference ranges of peripheral blood T-lymphocyte subsets values were proposed by the current study for healthy adults in Hunan, providing more clinical information.更多还原