Objective To construct recombinant eukaryotic expression plasmid named pEGFP-N2-Wnt3a. Methods Total RNA was extracted from SD Rat's spinal cord and the Wnt3a gene was amplified by reverse transcription-polymerase chain reaction(RT-PCR). By using gene recombination technique, Rat Wnt3a cDNA was inserted into retroviral vector pEGFP-N2. The recombinant plasmid was identified by a pair of specified primers containing the restriction sites of XhoI and EcoRⅠ. Results The Wnt3a gene was obtained by RT-PCR, the r...
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