【摘要】 目的建立小鼠脑组织中视黄酸的LC-MS/MS分析方法。方法通过电喷雾离子化,采用多反应检测方式进行正离子检测,用于定量分析的检测离子为301.1→123.1。采用Agilent SB-C18(100 mm×2.1 mm,1.8μm)柱分离,以乙腈-0.1%甲酸水溶液为流动相,梯度洗脱进行分析。结果视黄酸与其他成分分离良好。在0.92~9.20 ng/g(R2=0.993 7)范围内线性关系良好;平均加样回收率分别为93.5%、96.7%、103.1%,RSD分别为8.7%、6.5%、5.9%(n=3);检测限为0.2 ng/g。结论视黄酸检出时间在10 min内,且脑组织中内源性物质无干扰,方法快速、准确、专属性好,适于脑组织中视黄酸检测。更多还原

【Abstract】 Objective The aim of this study was to establish a liquid chromatography-tandem mass spectrum(LC-MS/MS) methodology for detecting retinoic acid in mice brain tissue. Methods Multiple-reaction-monitoring(MRM) mode was utilized in electrospray ionization. The positive electric spray ionization mode was adopted. The ions for quantitative analysis of retinoic acid were 301.1→123.1. Agilent SB-C18(100×2.1mm, 1.8μm) column was adopted and the mobile phase was aqueous solution of acetonitrile and 0.1% formic acid. Results The separation of retinoic acid from other components was good. The calibration curves were linear between 0.92-9.20 ng/g(R2=0.9937). The average recovery rates were 93.5%, 96.7%, and 103.1% respectively, while RSD were 8.7%, 6.5%, and 5.9% respectively(n=3). Conclusion Retinoic acid was able to be detected in ten minutes and endogenous substances did not interfere with the seperation. This methodology was suitable for the detection of retinoic acid in mice brain tissue. The methodology was quick, accurate with high specificity.更多还原