【摘要】 目的研究巨噬细胞RAW264.7诱导共培养HL60细胞凋亡的作用机理。方法用佛波脂(phorbol-1,2-myristate-1,3-acetate,PMA)、脂多糖(LPS)和γ-干扰素(IFN-γ)诱导培养的巨噬细胞RAW264.7,并与HL60细胞共培养12 h。采用MTT法、流式细胞计量术、DNA琼脂糖凝胶电泳及蛋白印迹技术,观察巨噬细胞与肿瘤细胞共培养体系中,RAW264.7细胞对HL60细胞的作用。结果与巨噬细胞RAW264.7共培养的HL60细胞存活率减少,表现出凋亡细胞特有的标志,即"G1亚峰"和DNA梯状电泳条带,在这一过程中还伴随有促凋亡基因P53和P21表达。结论诱导刺激巨噬细胞RAW264.7导致了共培养HL60细胞的凋亡。更多还原

【Abstract】 Objective To observe the effects of apoptosis of HL60 cells co-cultured with RAW264.7 macrophages. Methods RAW264.7 macrophages were stimulated with phobolmyristate-acetate(PMA), lipopolysaccharide(LPS) and interferon-γ(IFN-γ), then co-cultured with HL60 cells for 12 hours. The effects of RAW264.7 macrophages on HL60 cells were assayed by MTT, flow cytometry(FCM), DNA agarose gel electrophoresis and western blotting. Results HL60 cells co-cultured with RAW264.7 macrophages resulted in decreased cell viability, and evidently increased special expression of apoptotic cells, characterized by G1 sub-peak and DNA ladder shaped electrophoresis, and expression of promote-apoptosis-gene P53 and P21. Conclusion Inducible RAW264.7 macrophages can cause apoptosis of co-cultured HL60.更多还原