【摘要】 背景:天津大学材料学院利用仿生学方法制备的纳米羟基磷灰石/细菌纤维素复合支架材料,具有与天然骨相似的结构和性能。目的:研究纳米羟基磷灰石/细菌纤维素复合组织工程支架的细胞毒性和生物相容性。方法:1急性全身性毒性实验:将纳米羟基磷灰石/细菌纤维素材料浸提液与生理盐水分别注射至昆明小鼠腹腔,注射24,48,72 h记录小鼠体质量。2致敏实验:在日本大耳白兔背部皮下分别注射纳米羟基磷灰石/细菌纤维素材料浸提液与生理盐水,72 h内观察注射部位水肿及红斑情况,间隔14 d后再次行激发实验。3热源实验:在日本大耳白兔耳缘静脉注射纳米羟基磷灰石/细菌纤维素材料浸提液,注射后检测体温变化。4溶血实验:在稀释的兔抗凝血中分别加入纳米羟基磷灰石/细菌纤维素材料浸提液、生理盐水与蒸馏水。5将第3代兔骨髓间充质干细胞与纳米羟基磷灰石/细菌纤维素材料共培养,观察材料表面细胞增殖、生长及黏附状态。结果与结论:纳米羟基磷灰石/细菌纤维素复合支架材料无急性全身毒性、无致敏性、无热源反应、无溶血反应,该支架材料具有三维网络结构,骨髓间充质干细胞在材料表面生长、增殖及黏附良好,表明纳米羟基磷灰石/细菌纤维素复合支架材料具有良好的生物相容性与细胞相容性。更多还原

【Abstract】 BACKGROUND: Nano-hydroxyapatite/bacterial cellulose(n HA/BC) composite scaffolds prepared by nano-bionics method exhibit a structure and performance similar to natural bone.OBJECTIVE: To study the cytotoxicity and biocompatibility of n HA/BC composite scaffolds.METHODS:(1) Acute systemic toxicity test: n HA/BC leach liquor and normal saline were respectively injected intraperitoneally into Kunming mice. After 24, 48, 72 hours, the body mass of mice was recorded.(2) Sensitization test: n HA/BC leach liquor and normal saline were respectively injected subcutaneously into the back of Japanese white rabbits. With 72 hours of injection, edema and erythema were observed, and the experiment was done again at an interval of 14 days.(3) Pyrogen test: n HA/BC leach liquor and normal saline were respectively injected into the ear vein of Japanese white rabbits, and after injection, the body temperature of rabbits were determined.(4) Hemolysis test: Diluted rabbit anti-coagulant was added into n HA/BC leach liquor, normal saline and distilled water, respectively.(5) Passage 3 bone marrow mesenchymal stem cells from rabbits were co-cultured with n HA/BC materials, and then, cell proliferation, growth and adhesionon the material surface were observed.RESULTS AND CONCLUSION: n HA/BC composite scaffold was of no acute systemic toxicity, non-allergenic, no pyrogenic and hemolytic reactions; n HA/BC had a three-dimensional porous structure that was better for growth,proliferation and adhesion of rabbit bone marrow mesenchymal stem cells. These findings indicate that the n HA/BC composite scaffold has good biocompatibility and cytocompatibility.更多还原