【摘要】 背景:骨折愈合过程十分复杂,需要多种细胞因子的参与。目前研究较多的细胞因子有骨形态发生蛋白、成纤维细胞生长因子、转化生长因子β、血管内皮细胞生长因子和胰岛素样生长因子,但神经生长因子在骨折愈合过程中对血管内皮细胞生长因子的作用尚不明确。目的:观察神经生长因子对兔骨折愈合中血管内皮生长因子表达的影响。方法:实验建立标准兔桡骨骨折模型,分别用神经生长因子、神经生长因子单克隆抗体和生理盐水进行干预,即应用神经生长因子组、拮抗神经生长因子组和对照组。结果与结论:损伤后24,48 h和损伤后1,3,6,8周Western blot检测骨折端组织血管内皮生长因子蛋白的表达分析结果显示,3组各时间点血管内皮生长因子表达的关系为:应用神经生长因子组>对照组>拮抗神经生长因子组(P<0.05)。结果证实,在骨折愈合过程当中,应用神经生长因子可以促进血管内皮生长因子表达。更多还原

【Abstract】 BACKGROUND: The fracture healing process is very complex, involving a variety of cytokines. At present, the existing studies focus on bone morphogenetic protein, fibroblast growth factor, transforming growth factor β, vascular endothelial cell growth factor and insulin-like growth factor. The role of nerve growth factor on vascular endothelial cell growth factor during the fracture healing process remains unclear. OBJECTIVE: To investigate the effect of nerve growth factor on vascular endothelial growth factor expression during the process of fracture healing in rabbits. METHODS: Radial fracture model established in white rabbits were divided into three groups, they were treated with nerve growth factor, nerve growth factor monoclonal antibody, and saline respectively, serving as nerve growth factor group, antagonizing nerve growth factor group, and control group. RESULTS AND CONCLUSION: The vascular endothelial growth factor expression was detected with western blot analysis at 24 hours, 48 hours, 1 week, 3 weeks, 6 weeks, 8 weeks post-injury. The results showed that the expression level was the highest in nerve growth factor group, then in control group, and the lowest in antagonizing nerve growth factor group(P < 0.05). Experimental findings suggest that, nerve growth factor can promote the expression of vascular endothelial growth factor in the fracture healing process.更多还原