【摘要】 背景:建立客观标准的骨不连动物实验模型是骨不连的实验研究及探索骨不连治疗的必要条件。目的:为骨不连的实验研究建立一种客观的动物实验模型。方法:选取SD大鼠,在胫骨中段截断,用口腔科粘固粉充填器(3#或4#)灼烧骨折断端(包括骨膜),然后用0.5 mm克氏针钻入骨髓腔,固定骨折,2个月后经大体标本、病理组织学及放射学检查确定骨不连形成情况。结果与结论:大体标本观察、放射学检查及病理组织学检查均显示骨缺损区无骨性连接,为纤维瘢痕组织填充,骨端硬化。结果表明实验所建立的动物模型,具有骨不连的病理改变,符合骨不连的要求,是一种可靠而实用的实验性骨不连动物模型。更多还原

【Abstract】 BACKGROUND: Establishment of an objective and standard experimental animal model of bone non-union is necessary for experimental study and related treatment of non-union. OBJECTIVE: To establish an objective experimental animal model for the experimental study of non-union. METHODS: Sprague-Dawley rats were included in this study, the middle tibia was cut off, and fracture fragments(including periosteum) were seared with dental cement plugger(3# or 4#). Subsequently, bone marrow cavity was drilled using a 0.5 mm Kirschner wire, and the fractures were fixed. 2 months later, the specimens were detected by gross observation, histopathological detection and radiological examination to determine the nonunion formation. RESULTS AND CONCLUSION: The results of gross observation, histopathological detection and radiological examination showed that, no bony union was found at bone defect area, which was filled by fiber scar tissue, and bone end sclerosis was apparent. The animal models established in this study have pathological changes of bone non-union, which is in line with the requirements of bone non-union, so the model can be considered a reliable and practical animal model of bone non-union.更多还原