【摘要】 背景:自组装多肽类材料因其独特的设计及良好的生物相容性和可降解性在众多三维支架材料中脱颖而出。目的:综述RADA类离子互补型自组装多肽支架材料的结构和功能化设计,从细胞三维培养方面探讨多肽类材料作为细胞载体材料在细胞治疗中的应用前景。方法:由作者通过PubMed、Web of science数据库及CNKI数据库检索有关自组装多肽水凝胶的相关文献,检索词为"self-assembly peptide,tissue engineering;自组装多肽,组织工程",检索文献量总计224篇,纳入包含多肽材料设计、功能化多肽材料、多肽材料用于细胞三维培养方面的研究,最终纳入48篇。结果与结论:从物理结构角度讲,多肽材料可以在生理环境中自组装成具有纳米级纤维和较高孔隙率的水凝胶,最大程度上模拟细胞外基质的结构,保障细胞生存在一个真正的三维环境中。从生物功能角度讲,多肽材料可以根据不同需求复合特异性的生物活性短肽片断,赋予材料一定的细胞特异性,可以促进细胞的黏附、增殖或分化。更多还原

【Abstract】 BACKGROUND: Self-assembling peptide scaffolds have attracted much more attention among three dimensional biomaterials due to its biocompatibility, biodegradation, and tailor-made properties. OBJECTIVE: To review the structure and design of functionalized self-assembling RADA peptide, and the recent advances in the use of RADA self-assembling peptide for three-dimensional cell culture in cell therapy applications. METHODS: Total 224 literatures related to self-assembling peptide and tissue engineering from PubMed, Web of Science and CNKI databases were screened out for this review. The keywords were "self-assembly peptide, tissue engineering" in English and Chinese, respectively. Finally 48 of 224 articles about the design, fictionalization, and three-dimensional cell culture of peptide scaffolds were included in this review. RESULTS AND CONCLUSION: Self-assembling peptide could undergo spontaneous assembly into well-ordered interwoven nanofibers in water and rapidly form hydrogel, which physically mimics the architecture of natural extracellular matrix to ensure a real three-dimensional microenvironment for cells. In terms of bio-function, this material can be tailor-made with various bioactive short peptide motifs to promote cell adhesion, proliferation, and differentiation.更多还原