【摘要】 背景:前期实验发现掺锶的聚磷酸钙材料对单独培养内皮细胞或成骨细胞的行为有显著促进作用。目的:观察掺锶聚磷酸钙对共培养下成骨细胞与脐静脉内皮细胞行为和功能蛋白表达的影响。方法:取第3代人脐静脉内皮细胞与人成骨肉瘤细胞MG63以2∶1的浓度比接种于24孔板中,然后再分别加入掺锶聚磷酸钙、聚磷酸钙与羟基磷灰石,共培养7 d后。采用ELISA法检测细胞血管内皮生长因子、碱性成纤维细胞生长因子蛋白的表达,采用MTT法检测细胞活性。结果与结论:与聚磷酸钙与羟基磷灰石相比,在掺锶聚磷酸钙表面生长的细胞呈现更加良好的形态,细胞融合生长形成单层覆盖在材料表面,并且在材料表面有一定的跨度生长,说明掺锶聚磷酸钙材料能促进内皮细胞在其上黏附、伸展,有利于细胞的生长和增殖。掺锶聚磷酸钙组细胞分泌的血管内皮生长因子、碱性成纤维细胞生长因子明显高于聚磷酸钙组和羟基磷灰石组(P<0.05),说明掺锶聚磷酸钙可上调血管内皮生长因子、碱性成纤维细胞生长因子蛋白的表达。更多还原

【Abstract】 BACKGROUND: Our previous studies have shown that strontium-doped calcium polyphosphate containing low-dose strontium appears to have a significant effect on angiogenesis-related behaviors of monocultured umbilical vein endothelial cells and osteoblasts. OBJECTIVE: To investigate the effect of strontium-doped calcium polyphosphate on angiogenesis-related behaviors of umbilical vein endothelial cells and osteoblasts co-cultured, including cell adhesion, spreading, proliferation, as well as the protein secretion of vascular endothelial growth factor and basic fibroblast growth factor from co-culture system in vitro. METHODS: Human umbilical vein endothelial cells and osteoblastic cells(MG63) were utilized in this study. Cells from passage 3 were used for preparation of the cell-scaffold constructs. After placed in 24-well plate at a ratio of 2:1, human umbilical vein endothelial cells and MG63 cells were seeded onto strontium-doped calcium polyphosphate, calcium polyphosphate and hydroxyapatite scaffolds and co-cultured for 7 days. The vascular endothelial growth factor and basic fibroblast growth factor protein levels were determined through a double ligand enzyme-linked immunosorbent assay. The colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide assay was performed to quantify the effect of scaffolds on cell proliferation. RESULTS AND CONCLUSION: Compared with those on calcium polyphosphate and hydroxyapatite scaffolds, cells on strontium-doped calcium polyphosphate scaffolds attached and spread better with a significantly improved cell proliferation. More importantly, the vascular endothelial growth factor and basic fibroblast growth factor expressions were significantly higher in the strontium-doped calcium polyphosphate group than the other two groups(P < 0.05), indicating strontium-doped calcium polyphosphate can up-regulate levels of vascular endothelial growth factor and basic fibroblast growth factor proteins.更多还原