【摘要】 背景:早期受损的软骨细胞在体外培养时容易产生去分化,表型不稳,常需添加一定的生长因子。目的:观察胰岛素样生长因子1对成年兔创伤性关节炎早期关节软骨细胞体外增殖的促进作用。方法:采用改良Hulth法制备成兔创伤性关节炎模型,造模成功后无菌条件下片状切取股骨远端及胫骨近端,用消化培养法培养软骨细胞。将软骨细胞随机分为2组,对照组加入含体积分数10%胎牛血清因子的DMEM培养液培养;实验组在对照组的基础上加入100μg/L的胰岛素样生长因子1。通过细胞形态学、细胞计数、细胞活性检测胰岛素样生长因子1对创伤性关节炎关节软骨细胞增殖的影响。结果与结论:成功培养出早期创伤性关节炎兔软骨细胞,细胞多数为小细胞,形态有小梭形、小圆形及小多边形。苏木精-伊红染色显示实验组细胞数量多于对照组,MTT实验证实实验组细胞的吸光度值大于对照组(P<0.01)。结果提示,胰岛素样生长因子1能促进早期创伤性关节炎模型兔软骨细胞的体外增殖。更多还原

【Abstract】 BACKGROUND: The early damaged chondrocytes are susceptible to de-differentiate and exert unstable phenotype during the in vitro culture, thus needing some growth factors. OBJECTIVE: To observe the promotion effect of insulin-like growth factor 1 on the in vitro proliferation of chondrocytes in adult rabbits with traumatic arthritis. METHODS: Traumatic arthritis models of adult rabbits were established by using the modified Hulth method. After the models were successfully established, the distal femur and proximal tibia were harvested under sterile conditions, the chondrocytes were cultured. The cultured cells were divided into two groups: control group was cultured with Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, while experimental group was cultured with Dulbecco’s modified Eagle’s medium containing 100 μg/L insulin-like growth factor 1. The effect of insulin-like growth factor 1 on the proliferation of chondrocytes in adult rabbits with traumatic arthritis was determined through the cytomorphology, cell counting, and cell activity. RESULTS AND CONCLUSION: The chondrocytes in adult rabbits with traumatic arthritis were successfully cultured, the majority of cells were mini-cells, presenting small fusiform, round or polygonal shape. Hematoxylin-eosin staining showed that the number of cells in experimental group was higher than that in control group. MTT assay found that the absorbance of cells in experimental group was greater than that in control group(P < 0.01). Our findings indicate that, insulin-like growth factor 1 can promote the in vitro proliferation of chondrocytes in adult rabbits with traumatic arthritis.更多还原